[Purification and properties of cyclic nucleotide phosphodiesterase from uterine tissue]

N O Artem'ev, M N Garnovskaia, I L Dunler

|August 1, 1988

Abstract:

Cyclic nucleotide phosphodiesterase from calf myometrium has been purified to a homogeneous state for the first time, as can be evidenced from polyacrylamide gel electrophoresis data. The purification procedure included ion-exchange chromatography on DEAE-cellulose, high pressure liquid chromatography on TSK 545 DEAE and gel filtration through Toyopearl HW-55. The molecular mass of the enzyme as determined by gel filtration and polyacrylamide gel electrophoresis is 110 kD. The purified enzyme hydrolyzes cAMP and cGMP with Km = 30 microM and 18 microM, respectively.

Related Concept Videos

Articles linked to this work by shared authors, journal, and citation graph.

[Synthesis of visual rhodopsin in a cell-free translation system. II. Functional properties of recombinant rhodopsin and its mutant forms].

Bioorganicheskaia khimiia·1990

[Aminobenzoic acid derivatives as specific inhibitors of cyclic nucleotide phosphodiesterase in the rat uterus].

Ukrainskii biokhimicheskii zhurnal (1978)·1990

[The functional similarity of vertebrate rhodopsin and of a photosensitive pigment from the unicellular flagellate alga Chlamydomonas reinhardtii].

Zhurnal evoliutsionnoi biokhimii i fiziologii·1989

Related Experiment Videos

[Purification and properties of cyclic nucleotide phosphodiesterase from uterine tissue]

Related Concept Videos

[Synthesis of visual rhodopsin in a cell-free translation system. II. Functional properties of recombinant rhodopsin and its mutant forms].

[Aminobenzoic acid derivatives as specific inhibitors of cyclic nucleotide phosphodiesterase in the rat uterus].

[The functional similarity of vertebrate rhodopsin and of a photosensitive pigment from the unicellular flagellate alga Chlamydomonas reinhardtii].

[Peptide-activator of cyclic nucleotide phosphodiesterases in the rat and human uterus].

[The peptide activator of cyclic nucleotide phosphodiesterases: its effect on muscle contractile activity].

[Detection of peptide, an activator of cyclic nucleotide phosphodiesterases, in uterine tissue].

PPsAMP: A Novel Computational Framework for Short Antimicrobial Peptide Identification by Fusing Fine-Tuned Semantic and Physicochemical Features via Cross-Attention.

Fragment-Based Discovery of KLK6 and KLK7 Inhibitors.

Mechanism of Isonitrilase, a Nonheme Iron Decarboxylase Involved in the Biosynthesis of the Isonitrile Group in Peptides: A Computational Study.

Quality, chemical composition, and antimicrobial potential of Citrus peel essential oils against urinary tract infection pathogens.

Highly-Efficient Cellular Uptake and Membrane Permeability of Cell-Penetrating Peptides via Introduction of Homoarginine Residues.

Techniques for Accelerating the Understanding of PROTAC-Based Ternary Complex.

Related Experiment Videos

[Purification and properties of cyclic nucleotide phosphodiesterase from uterine tissue]

Abstract:

Related Concept Videos

Related Articles