In vivo electron paramagnetic resonance oximetry and applications in the brain

Molecular oxygen (O₂) is essential to brain function and mechanisms necessary to regulate variations in delivery or utilization of O₂ are crucial to support normal brain homeostasis, physiology and energy metabolism. Any imbalance in cerebral tissue partial pressure of O₂ (pO₂) levels may lead to pathophysiological complications including increased reactive O₂ species generation leading to oxidative stress when tissue O₂ level is too high or too low. Accordingly, the need for oximetry methods, which assess cerebral pO₂in vivo and in real time, is imperative to understand the role of O₂ in various metabolic and disease states, including the effects of treatment and therapy options. In this review, we provide a brief overview of the common in vivo oximetry methodologies for measuring cerebral pO₂. We discuss the advantages and limitations of oximetry methodologies to measure cerebral pO₂in vivo followed by a more in-depth review of electron paramagnetic resonance oximetry spectroscopy and imaging using several examples of current electron paramagnetic resonance oximetry applications in the brain.