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THE BIOLOGICAL ACTIVITIES OF HYPERICUM PERFORATUM L
Gulten Okmen1, Neslihan Balpınar2
1Department of Biology, Faculty of Science, University of Mugla Sitki Kocman, 48000 Mugla, Turkey.
Background:
Mastitis reduces milk yield and alters milk composition. Antibiotics are widely used in the treatment of the disease. However, this widespread use of antibiotics causes both antibiotic residues in milks and antibiotic resistance developed in bacteria. Today's researches are focused on discovering and using new antibiotics against bacteria.
Objective:
The aim of this work was to discover the antibacterial effects of Hypericum perforatum L. extracts against mastitis pathogens, and its other biological activities.
Material And Methods:
Kirby-Bauer assay was applied to the extracts. The other antibacterial activity was MIC for plant extracts. The non-enzymatic antioxidant activity was found using 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH).
Results:
The extract was showed maximum inhibition zone against two bacteria (Coagulase-negative Staphylococci-33 and 37; CNS 33 and 37), and the zone was 17 mm. A bacterium (CNS - 22) showed the lowest sensitivity to 812.5 μg/mL concentration. In addition, the extract was tried against the stable DPPH for antioxidant activity. As a result, the extract showed a strength antioxidant activity. Trolox equivalent is 0.83 mM.
Conclusion:
The extract of Hypericum perforatum have antibacterial, antioxidant and antimutagenic potentials.
Insights
Hypericum perforatum extract shows strong antibacterial and antioxidant effects against mastitis pathogens. This natural compound offers a promising alternative to conventional antibiotics, addressing concerns of residues and resistance.
Area of Science:
- Phytochemistry
- Microbiology
- Pharmacology
Background:
- Mastitis negatively impacts milk production and composition.
- Antibiotic overuse in mastitis treatment leads to residues and bacterial resistance.
- Novel antimicrobial agents are crucial for combating resistant mastitis pathogens.
Purpose of the Study:
- To investigate the antibacterial efficacy of Hypericum perforatum L. extracts against mastitis-causing bacteria.
- To evaluate other biological activities, including antioxidant potential, of Hypericum perforatum L. extracts.
Main Methods:
- Antibacterial activity was assessed using Kirby-Bauer disk diffusion and Minimum Inhibitory Concentration (MIC) assays.
- Antioxidant capacity was determined using the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) assay.
Main Results:
- Hypericum perforatum extract exhibited significant antibacterial activity, with a maximum inhibition zone of 17 mm against specific Coagulase-negative Staphylococci strains.
- The extract demonstrated potent antioxidant activity, with a Trolox equivalent antioxidant capacity of 0.83 mM.
Conclusions:
- Hypericum perforatum extract possesses notable antibacterial and antioxidant properties.
- These findings suggest Hypericum perforatum as a potential natural therapeutic agent for mastitis.
- Further research into its antimutagenic potential is warranted.
