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Published on: February 17, 2023
Application of platelet derived growth factor-BB and diabetic wound healing: the relationship with oxidative events
Sibel Gökşen1, Barbaros Balabanlı1, Şule Coşkun-Cevher1
1a Department of Biology, Faculty of Science , Gazi University , Ankara , Turkey.
Abstract:
The reasons that cause delay in wound healing in diabetes are a decrease in the level of growth factors secretion, an increase in the destruction of growth factors and in oxidative stress. Platelet derived growth factor (PDGF) is one of the important growth factors that play a role in all phases of wound healing. This study investigates time-dependent effects of topically PDGF-BB administration on oxidative events on the healing of dorsolateral-excisional wounds in diabetic rats. Forty-two female Wistar-albino rats with streptozotocin-induced diabetes were divided into four groups: control group, untreated group, chitosan-treated group, chitosan + PDGF-BB-treated group. Two identical full-thickness excisional skin wounds were made under anaesthesia in all rats except for the control group. In the PDGF-BB-treated and chitosan-treated groups, the wounds were treated topically PDGF-BB (7 ng/mL, single daily dose) and blank chitosan gel (equal amount) after wounding, respectively. After these administrations, on day 3 and day 7 of wound healing, rats were sacrificed. Thiobarbituric acid reactive substances, glutathione, nitric oxide, ascorbic acid levels, and superoxide dismutase activity in wound tissues were spectrophotometrically measured. PDGF-BB administration significantly increased TBARS levels and non-enzymatic antioxidant levels in early phase of diabetic wound healing. PDGF-BB dramatically reduced NOx levels on day 3 and sharply increased NOx levels on day 7 of wound healing. Consequently, PDGF-BB administration can be effective on oxidative balance in the early phase of diabetic wound healing.
Insights
Platelet-derived growth factor (PDGF)-BB topical administration impacts oxidative balance in diabetic wound healing. It increased early-phase oxidative stress markers and altered nitric oxide levels, suggesting a role in managing diabetic wound healing.
Area of Science:
- Biomedical Science
- Wound Healing Research
- Diabetic Complications
Background:
- Diabetic wound healing is impaired by reduced growth factors, increased growth factor degradation, and oxidative stress.
- Platelet-derived growth factor (PDGF) is crucial for all wound healing phases.
- Understanding PDGF's role in diabetic wound oxidative events is vital.
Purpose of the Study:
- To investigate the time-dependent effects of topical Platelet-derived growth factor (PDGF)-BB on oxidative events during diabetic wound healing in rats.
- To assess the impact of PDGF-BB on markers of oxidative stress and antioxidant capacity in wound tissues.
Main Methods:
- Streptozotocin-induced diabetic rats with excisional wounds were divided into control, untreated, chitosan-treated, and chitosan + PDGF-BB-treated groups.
- Topical PDGF-BB (7 ng/mL) or chitosan gel was applied daily.
- Wound tissues were analyzed on days 3 and 7 for thiobarbituric acid reactive substances (TBARS), glutathione, nitric oxide (NOx), ascorbic acid, and superoxide dismutase (SOD) activity.
Main Results:
- PDGF-BB administration significantly increased TBARS and non-enzymatic antioxidant levels in the early phase of healing.
- PDGF-BB markedly reduced NOx levels on day 3 and sharply increased them on day 7.
- Chitosan gel alone did not show significant effects compared to the untreated group (data not shown).
Conclusions:
- Topical PDGF-BB influences oxidative balance during the early stages of diabetic wound healing.
- PDGF-BB's modulation of oxidative stress markers and nitric oxide suggests a potential therapeutic role.
- Further research is warranted to optimize PDGF-BB application for diabetic wound management.
