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Related Experiment Videos

Retinoic acid and thyroid hormone induce gene expression through a common responsive element.

K Umesono1, V Giguere, C K Glass

  • 1Howard Hughes Medical Institute, La Jolla, California.

Nature
|November 17, 1988
PubMed
Summary

Retinoic acid receptors can activate gene expression via thyroid hormone response elements. This suggests overlapping gene networks regulated by retinoic acid and thyroid hormones in vertebrates.

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Area of Science:

  • Molecular Endocrinology
  • Gene Regulation
  • Cellular Biology

Background:

  • Steroid receptors form a superfamily of ligand-inducible proteins, including thyroid hormone and retinoic acid receptors.
  • These receptors regulate gene expression by binding to hormone-response elements.
  • Understanding retinoic acid receptor mechanisms requires identifying functional retinoic acid responsive elements.

Purpose of the Study:

  • To investigate if retinoic acid receptors can activate gene expression through thyroid hormone response elements.
  • To explore the functional overlap between retinoic acid and thyroid hormone signaling pathways.

Main Methods:

  • Utilizing cloned complementary DNA for human retinoic acid receptor expression.
  • Employing F9 teratocarcinoma cells to study endogenous retinoic acid receptor activity.

Related Experiment Videos

  • Assessing gene expression from promoters fused to natural or synthetic thyroid hormone response elements.
  • Performing in vitro translation of human retinoic acid receptor cDNA to study DNA binding affinity.

    • Main Results:

    • Human retinoic acid receptors (expressed or endogenous) activated gene expression from thyroid hormone response elements.
    • In vitro translated human retinoic acid receptor demonstrated high-affinity binding to thyroid hormone response elements.
    • Demonstrated functional cross-activation between retinoic acid and thyroid hormone receptor pathways.

    Conclusions:

    • Retinoic acid receptors can functionally interact with thyroid hormone response elements.
    • This interaction implies that retinoic acid and thyroid hormones may control shared gene networks.
    • These overlapping networks are potentially crucial for vertebrate development and homeostasis.