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Updated: Mar 2, 2026
Synthesis of an Intein-mediated Artificial Protein Hydrogel
Published on: January 27, 2014
Spontaneous and efficient adsorption of lysozyme from aqueous solutions by naturally polyanion gel beads
Jiwei Li1, Yang Si2, Cunyi Zhao2
1MIIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage, School of Chemistry and Chemical Engineering, Harbin Institute of Technology, Harbin 150001, China; Fiber and Polymer Science, University of California, Davis, CA 95616, USA.
Abstract:
In this study, polyanionic alginate gel beads crosslinked by Ca2+ and glutaraldehyde have demonstrated a strong electrostatic interaction with specific proteins. Due to the naturally abundant carboxyl groups, the prepared alginate gel beads exhibited a relatively superior integrated adsorption performance toward lysozyme, including a superior adsorption capacity of 213mgg-1, fast adsorption equilibrium within 12h, good selectivity, and good reversibility. Compared with other protein adsorbents, the as-prepared adsorptive beads have the advantages of excellent adsorption performance, easy to prepare, convenient, efficient, reliable and environment-friendly to apply, which can serve as a more sustainable material in protein separation and purification.
