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Morphology-Based Distinction Between Healthy and Pathological Cells Utilizing Fourier Transforms and Self-Organizing Maps
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Bimodal morphological analyses of native and engineered tissues.

Sasan Jalili-Firoozinezhad1, Ivan Martin1, Arnaud Scherberich1

  • 1Department of Biomedicine, University Hospital Basel, University of Basel, Hebelstrasse 20, CH-4031 Basel, Switzerland; Department of Biomedical Engineering and of Surgery, University Hospital Basel, University of Basel, Hebelstrasse 20, CH-4031 Basel, Switzerland.

Materials Science & Engineering. C, Materials for Biological Applications
|May 10, 2017
PubMed
Summary

This study introduces Histo-SEM, a novel technique combining scanning electron microscopy with histology. Histo-SEM reveals detailed tissue architecture, aiding in the assessment of native and engineered tissues.

Keywords:
Extracellular matrixHistologyMorphologyScanning electron microscopyTissue structure

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Microscopy

Background:

  • Assessing native and engineered tissue morphology is crucial for understanding development and structure-function relationships.
  • Conventional histology and immunohistochemistry offer limited architectural insights.
  • Scanning electron microscopy (SEM) provides high resolution but lacks direct correlation with histological findings.

Purpose of the Study:

  • To establish a Histo-SEM technique for evaluating morpho-architectural properties of native and engineered tissues.
  • To integrate SEM with histological processing of paraffin-embedded sections.
  • To enable detailed assessment of tissue maturation and organization.

Main Methods:

  • Developed Histo-SEM by analyzing paraffin-embedded tissue sections prepared for histology.
  • Applied Histo-SEM in parallel with histological/immunohistochemical staining.
  • Examined cartilaginous, bone, and fibrous tissues, both native and engineered.

Main Results:

  • Histo-SEM revealed collagen fiber diameter and orientation for cartilage maturation assessment.
  • Identified nanoscale-dense matrix structures indicative of bone tissue/osteoid formation.
  • Quantified tissue integration and vascularization by analyzing collagen fiber density and vessel wall thickness in fibrous tissue.

Conclusions:

  • Histo-SEM integrates bimodal morphological assessments for native and engineered tissues.
  • The technique provides complementary qualitative and quantitative parameters for structural organization and maturation.
  • Histo-SEM enhances the evaluation of tissue development and potential structure-function relationships.