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Water-in-Oil Microemulsions for Protein Delivery: Loading Optimization and Stability
Diego R Perinelli1, Marco Cespi1, Stefania Pucciarelli2
1School of Pharmacy, University of Camerino, via Gentile III da Varano, 62032 Camerino, MC, Italy.
Current Pharmaceutical Biotechnology
|May 10, 2017
Summary
This study optimized microemulsions for enhanced protein delivery, achieving high protein loading and demonstrating long-term formulation stability for therapeutic applications.
Area of Science:
- Pharmaceutical Sciences
- Biotechnology
- Materials Science
Background:
- Microemulsions offer enhanced bioavailability for therapeutic proteins and peptides.
- Limited data exists on protein loading and stability in microemulsions using approved pharmaceutical excipients.
- This study addresses this gap using a model protein.
Purpose of the Study:
- To optimize a ternary microemulsion system (water/ethyl oleate/Span® 80-Tween® 80).
- To determine the protein loading capacity of the optimized system.
- To assess the long-term stability of the protein-loaded microemulsion.
Main Methods:
- Optimization using Central Composite Design.
- Characterization via dynamic light scattering, rheology, and microscopy.
- Protein loading determination and stability assessment over six months.
- Fluorescence spectroscopy to analyze protein structure.
Main Results:
- Protein incorporation decreased aqueous solubility but maintained formulation stability for six months.
- Native-like structure of the recovered bovine serum albumin (BSA) was confirmed.
- High protein loading capacity was achieved.
Conclusions:
- Feasibility of preparing microemulsions with high protein content demonstrated.
- Optimized microemulsions exhibit long-term stability for protein delivery.
- Potential for enhanced bioavailability of therapeutic proteins and peptides.
