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Detection of Leishmania within sand flies by kinetoplast DNA hybridization
W O Rogers1, P F Burnheim, D F Wirth
1Department of Tropical Public Health, Harvard School of Public Health, Boston, Massachusetts 02115.
The American Journal of Tropical Medicine and Hygiene
|November 1, 1988
Abstract:
A kinetoplast DNA hybridization probe method was used to detect Leishmania within sand flies and to distinguish it from the non-pathogenic flagellate, Endotrypanum. Eighty-one sand flies (74 Lutzomyia umbratilis, 1 Lu. anduzei, and 6 Lu. shannoni) collected outside Manaus, Brazil were dissected. Forty-four of these were found to be infected with flagellates, and 2 hybridized with a Leishmania braziliensis probe. Thirty-three of sixty-one flies reprobed with an Endotrypanum probe were positive.