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An Optimized Enrichment Technique for the Isolation of Arthrobacter Bacteriophage Species from Soil Sample Isolates
Published on: April 9, 2015
Isolation and Characterization of an Aeromonas punctata Bacteriophage
Cheng Kai1,2, Zhang Denglan1, Deng Jingxuan2
1Key Laboratory of Ecological Remedlation of Lakes and Rivers and Algal Utilization of Hubei Province, College of Resources and Environmental Engineering, Hubei University of Technology, Wuhan, Hubei, 430068, P.R. China.
Abstract:
An Aeromonas punctata bacteriophage, named as DH1, was isolated from East Lake, Wuhan city, China. Morphologically, phage DH1 showed a typical Myoviridae structure consisting of an isometric head (50 nm in diameter) and a visible tail. The bacteriophage had a latent period of about 90 minutes and an average burst size of about 125 PFU•Cell-1. Restriction enzyme pattern of the bacteriophage's genome showed that the genome is a double-stranded DNA and about 34kb in size. The sequenced genomic fragments showed highly similarities to gp04 and gp16 sequence of other Myoviridae bacteriophages at protein level.
