Related Experiment Video
Updated: Mar 2, 2026
12:25
Counting Proteins in Single Cells with Addressable Droplet Microarrays
Published on: July 6, 2018
9.0K
High-Density Droplet Microarray of Individually Addressable Electrochemical Cells.
Huijie Zhang1, Tobias Oellers2, Wenqian Feng3
1Center for Electrochemical Sciences-CES, Ruhr-Universität Bochum , Universitätsstrasse 150, 44780 Bochum, Germany.
Analytical Chemistry
|May 11, 2017
Summary
We developed an electrochemical droplet microarray (eDMA) for high-throughput screening. This novel platform enables sensitive, label-free detection of molecules in individual droplets, expanding screening capabilities.
Area of Science:
- Electrochemistry
- Microarray Technology
- High-Throughput Screening
Background:
- Microarray platforms often rely on optical detection, limiting their application scope.
- Electrochemical methods offer label-free, quantitative detection and individual compartment analysis, but are underutilized in high-throughput screening.
Purpose of the Study:
- To develop a high-density, individually addressable electrochemical droplet microarray (eDMA).
- To enable label-free electrochemical detection of redox-active molecules in nanoliter droplets for high-throughput screening.
Main Methods:
- Constructed an eDMA with orthogonal band microelectrodes forming three-electrode systems.
- Functionalized the electrode array with a patterned hydrophobic-hydrophilic polymer to create spatially separated nanoliter droplets.
- Utilized electrochemical characterization to assess droplet separation and electrode accessibility.
Main Results:
- Demonstrated detection of redox-active reporter molecules irrespective of electrochemical reversibility.
- Confirmed spatial separation of nanoliter droplets by hydrophobic boundaries.
- Showcased accessibility of the electrode system to redox-active molecules through hydrophilic patterns.
Conclusions:
- The eDMA technology enables high-throughput screening with sequential electrochemical read-out.
- This platform combines the advantages of droplet microarrays with sensitive electrochemical detection.
- Opens new avenues for biochemical and living cell screening applications.
