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MiR-486 regulates cardiomyocyte apoptosis by p53-mediated BCL-2 associated mitochondrial apoptotic pathway

Yuhan Sun1, Qiang Su1, Lang Li2

  • 1Department of Cardiology, The First Affiliated Hospital of Guangxi Medical University, Nanning, 530021, China.

BMC Cardiovascular Disorders
|May 11, 2017
PubMed
Abstract

Insights

MicroRNA-486 (miR-486) protects heart cells by inhibiting the p53-activated BCL-2 mitochondrial pathway. Upregulating miR-486 reduces cardiomyocyte apoptosis, offering a potential therapeutic strategy for heart disease.

Area of Science:

  • Cardiology
  • Molecular Biology
  • Cell Biology

Background:

  • Cardiomyocyte apoptosis is a critical factor in various heart diseases.
  • Understanding the molecular mechanisms regulating cardiomyocyte apoptosis is essential for developing effective treatments.

Purpose of the Study:

  • To investigate the role of microRNA-486 (miR-486) in regulating cardiomyocyte apoptosis.
  • To elucidate the involvement of the p53-activated BCL-2 associated mitochondrial pathway in miR-486's function.

Main Methods:

  • Primary cardiomyocytes were transfected with miR-486 mimics or inhibitors.
  • Hydrogen peroxide (H2O2) was used to induce apoptosis in cardiomyocytes.
  • Flow cytometry, TUNEL assay, RT-PCR, and Western blot were employed to assess apoptosis rates and molecular changes.

Main Results:

  • miR-486 overexpression significantly reduced p53, Bbc3, and cleaved caspase-3 expression while increasing BCL-2 levels.
  • Overexpression of miR-486 led to a significant decrease in cardiomyocyte apoptosis.
  • Silencing miR-486 resulted in an elevated rate of cardiomyocyte apoptosis.

Conclusions:

  • miR-486 regulates cardiomyocyte apoptosis through the p53-mediated BCL-2 associated mitochondrial pathway.
  • Increasing miR-486 expression in cardiomyocytes can protect them by inhibiting this apoptotic pathway.
  • miR-486 represents a potential therapeutic target for mitigating heart disease-related cardiomyocyte loss.
Keywords:
ApoptosisBCL-2 pathwayCardiomyocyteP53miR-486

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