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Related Concept Videos

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Three-Dimensional Microscopy in Microbiology

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Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
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Three-dimensional Rendering and Analysis of Immunolabeled, Clarified Human Placental Villous Vascular Networks
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Redefining 3Dimensional placental membrane microarchitecture using multiphoton microscopy and optical clearing.

Lauren Richardson1, Gracie Vargas2, Tyra Brown2

  • 1Department of Obstetrics & Gynecology, Division of Maternal-Fetal Medicine & Perinatal Research, Galveston, TX, USA.

Placenta
|May 11, 2017
PubMed
Summary

Advanced microscopy revealed structural changes like cell shedding and gaps in human placental membranes during pregnancy. This imaging technique offers new insights into normal and abnormal childbirth processes.

Keywords:
AmniochorionEpithelial sheddingMembrane ruptureMultiphoton microscopyNonlinear optical microscopyOptical clearingPlacentaPregnancy and parturitionSecond harmonic generation microscopy

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Area of Science:

  • Reproductive biology
  • Biomedical imaging
  • Histology

Background:

  • Human placental membranes undergo continuous remodeling throughout gestation to accommodate uterine growth.
  • Previous microscopic methods faced limitations in detailing cellular and layer-specific alterations.
  • Understanding these structural changes is crucial for comprehending parturition.

Purpose of the Study:

  • To characterize the 3D multilayer organization of placental membranes using advanced imaging techniques.
  • To overcome methodological limitations in visualizing microscopic changes within the membranes.

Main Methods:

  • Utilized multiphoton autofluorescence microscopy (MPAM) and second harmonic generation (SHG) microscopy.
  • Employed tissue optical clearing with 2,2'-thiodiethanol for deeper imaging.
  • Collected placental membrane biopsies from term, not-in-labor cesarean deliveries.

Main Results:

  • MPAM and SHG visualized epithelial topography and collagen organization in multiple matrix layers.
  • Observed epithelial shedding and gaps in term amnion layers.
  • Optical clearing enhanced visualization of collagen, mesenchymal cells, and decidua morphology.

Conclusions:

  • MPAM-SHG microscopy effectively studied the multi-layered placental membranes, revealing structural changes.
  • This advanced imaging approach can be applied to study uterine tissues and understand parturition events.