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Updated: Mar 2, 2026

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

Published on: September 20, 2019

A robust in vitro model for trans-lymphatic endothelial migration.

Yanbao Xiong¹, C Colin Brinkman¹, Konrad S Famulski²

¹Center for Vascular and Inflammatory Diseases, University of Maryland School of Medicine, Baltimore, USA.

Scientific Reports

|May 11, 2017

Summary

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This summary is machine-generated.

Researchers developed a new in vitro model to study how leukocytes migrate across lymphatic endothelial cells (LECs). This system reveals directional T cell migration towards chemoattractants, advancing our understanding of immune cell trafficking.

Area of Science:

Immunology
Cell Biology
Vascular Biology

Background:

Trans-endothelial migration (TEM) is critical for leukocyte circulation and immune surveillance.
While TEM across blood endothelial cells (BEC) is well-studied, lymphatic endothelial cell (LEC) TEM remains less understood.
Understanding lymphatic TEM is crucial for immune cell trafficking and disease progression.

Purpose of the Study:

To establish and validate an in vitro system for studying trans-lymphatic endothelial migration (TEM).
To investigate the directional migration patterns of various leukocyte subsets across lymphatic endothelium.
To explore factors influencing leukocyte TEM across LECs.

Main Methods:

Development of an in vitro model using primary mouse and human LECs, and the SVEC4-10 cell line.

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Assessment of Lymphocyte Migration in an Ex Vivo Transmigration System

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Evaluation of leukocyte transmigration in response to chemoattractants and varying conditions.

Assessment of T cell, macrophage, and dendritic cell migration patterns.

Main Results:

T cells demonstrated enhanced, unidirectional TEM from the basal to apical surface across LECs, unlike migration across BECs.
Leukocyte TEM across LECs was chemoattractant-driven, dose-dependent, and influenced by stromal factors.
Activated/memory CD4 T cells, macrophages, and DCs showed vectorial migration, but CD8 T cells did not.

Conclusions:

The established in vitro system accurately models lymphatic TEM and its influencing factors.
Leukocyte migration across lymphatic endothelium is a directed, chemoattractant-mediated process.
This model provides a versatile platform for high-throughput, reproducible studies of lymphatic biology and disease.