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Techniques for assessing canine mononuclear phagocyte function as part of an immunotoxicologic evaluation
P A Thiem1, L K Halper, J C Bloom
1Department of Experimental Pathology, Smith Kline and French Laboratories, King of Prussia, PA 19406-0939.
International Journal of Immunopharmacology
|January 1, 1988
Summary
Researchers developed new methods to assess dog immune system function, crucial for toxicologic studies. These techniques improve the evaluation of drug and chemical impacts on the mononuclear phagocyte system in dogs.
Area of Science:
- Veterinary immunology
- Toxicology
- Immunotoxicology
Background:
- Dogs are vital models in toxicologic research.
- Assessing immunotoxicity in dogs lacks practical methods.
- The mononuclear phagocyte system (MPS) is key in toxicologic responses.
Purpose of the Study:
- To adapt and validate practical techniques for assessing canine peripheral blood monocyte (PBM) function.
- To establish assays for key immunotoxicologic endpoints in dogs.
- To enhance the utility of dogs as models for toxicologic investigations.
Main Methods:
- Compared methods for isolating PBM, optimizing a technique for high purity and yield (>90%).
- Developed assays for antibody-dependent cellular cytotoxicity (ADCC), prostaglandin E2 (PGE2), and superoxide anion (O2-) production.
- Validated an interleukin-1 (IL-1) assay using EL-4 mouse thymoma cells.
Main Results:
- An optimized PBM isolation technique yielded >90% purity and improved recovery.
- Established methods for measuring ADCC, PGE2, O2-, and IL-1 production in canine PBM.
- The developed assays are suitable for assessing MPS function in dogs.
Conclusions:
- Practical methods for assessing canine PBM function are now available.
- These assays provide tools to evaluate drug and chemical effects on the canine MPS.
- The study enhances the dog's role as a model for immunotoxicologic studies.