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Annexin A2 functions downstream of c‑Jun N‑terminal kinase to promote skin fibroblast cell migration

Youpei Wang1, Xinmei Wu2, Qing Wang3

  • 1Clinical Examination Center, The Affiliated Eye Hospital of Wenzhou Medical University, Wenzhou, Zhejiang 325000, P.R. China.

Molecular Medicine Reports
|May 11, 2017
PubMed

Insights

Diabetes mellitus (DM) impairs skin wound healing by altering skin proteins. This study identified specific protein changes and found annexin A2 promotes fibroblast migration, potentially aiding wound repair in diabetic patients.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Dermatology

Background:

  • Delayed skin wound healing is a common complication of diabetes mellitus (DM).
  • The molecular mechanisms underlying impaired wound healing in DM are not fully understood.
  • Proteomic analysis offers a way to investigate systemic changes in disease states.

Purpose of the Study:

  • To identify proteomic alterations in the skin of a rat model with type 1 diabetes.
  • To explore the functional role of identified differentially expressed proteins in skin wound healing.
  • To investigate the potential involvement of annexin A2 in fibroblast migration.

Main Methods:

  • A type 1 diabetes mellitus rat model was induced using streptozotocin injections.
  • Proteomic analysis of normal and diabetic rat skin was performed using two-dimensional electrophoresis.
  • Protein identification was achieved through liquid chromatography/mass spectrometry.
  • Fibroblast cell migration assays were conducted with and without annexin A2 overexpression and JNK inhibition.

Main Results:

  • Diabetes mellitus induced 36 and repressed 41 differentially expressed proteins in rat skin.
  • Altered proteins were involved in critical cellular processes like metabolism, cytoskeleton regulation, and signal transduction.
  • Overexpression of annexin A2 enhanced human skin fibroblast cell migration.
  • Annexin A2 appears to function downstream of c-Jun N-terminal kinase (JNK) in regulating cell migration.

Conclusions:

  • This study elucidates potential proteomic changes in skin tissues affected by diabetes mellitus.
  • Annexin A2 plays a positive functional role in fibroblast cell migration, suggesting a therapeutic target for diabetic wound healing.
  • Understanding these molecular mechanisms can lead to improved treatments for diabetic skin complications.

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