Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.7K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.7K
Regulation of Angiogenesis and Blood Supply01:24

Regulation of Angiogenesis and Blood Supply

3.8K
Rapidly dividing tumors, embryos, and wounded tissues require more oxygen than usual, lowering the oxygen concentration in the blood. At low oxygen or hypoxic conditions, an oxygen-sensitive transcription factor called the hypoxia-inducible factor 1 or HIF1 is activated. HIF1 is a dimeric protein of alpha (ɑ) and beta (β) subunits.  Under optimal oxygen conditions, HIF1β is present in the nucleus while HIF1ɑ remains in the cytosol. HIF1ɑ is hydroxylated by prolyl...
3.8K
TGF - β Signaling Pathway01:16

TGF - β Signaling Pathway

10.8K
The TGF-β signaling pathway regulates cell growth, differentiation, adhesion, motility, and development. TGF-β ligands that induce TGF-β signaling are synthesized in their latent form. Several proteases or cell surface receptors such as integrins act upon the latent form, releasing the active ligand. There are three types of mammalian TGF-βs: (TGF-β1, TGF-β2, and TGF-β3) that bind as homodimers or heterodimers to TGF-β receptors. The TGF-β receptors...
10.8K
Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

3.6K
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
3.6K
Intracellular Signaling Affects Focal Adhesions01:17

Intracellular Signaling Affects Focal Adhesions

3.7K
Integrins act both as extracellular input receivers and as intracellular processing activators. As their name suggests, integrins are entirely integrated into the membrane structure. Their hydrophobic membrane-spanning regions interact with the phospholipid bilayer's hydrophobic region. These membrane receptors provide extracellular attachment sites for effectors like hormones and growth factors. They activate intracellular response cascades when their effectors are bound and active.
Some...
3.7K
IP3/DAG Signaling Pathway01:11

IP3/DAG Signaling Pathway

15.3K
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the  phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and...
15.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Retrospective Cohort Study of the Clinical Presentation and Visual Outcomes of Blepharoptosis Treatment.

Nigerian journal of clinical practice·2026
Same author

Extended follow-up of palbociclib with fulvestrant or letrozole for endocrine-sensitive, hormone receptor-positive/HER2-negative advanced breast cancer in the PARSIFAL trial.

ESMO open·2025
Same author

Perineural Invasion Exhibits Traits of Neurodegeneration.

Journal of dental research·2025
Same author

Novel Genetic Determinants of Dental Maturation in Children.

Journal of dental research·2022
Same author

Use of smartphones as a camera system for flexible cystoscopy during COVID-19 outbreak.

Annals of the Royal College of Surgeons of England·2022
Same author

Implementing reference systems for thyroid function tests - A collaborative effort.

Clinica chimica acta; international journal of clinical chemistry·2021

Related Experiment Video

Updated: Mar 2, 2026

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

8.0K

IGF-1 Mediates EphrinB1 Activation in Regulating Tertiary Dentin Formation.

S Matsumura1, A Quispe-Salcedo2, C M Schiller2

  • 11 Department of Oral and Maxillofacial Radiology, University of Connecticut Health Center, School of Dental Medicine, Farmington, Connecticut, USA.

Journal of Dental Research
|May 11, 2017
PubMed
Summary
This summary is machine-generated.

The insulin-like growth factor 1 (IGF-1)/ephrinB1 axis is crucial for tertiary dentin formation after tooth injury. This pathway regulates odontoblast differentiation and mineral density, offering potential for regenerative pulp therapy.

Keywords:
cell signalingdentinogenesishistochemistryodontogenesispulp biologystem cell(s)

More Related Videos

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
08:43

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Published on: April 3, 2018

10.4K
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

2.5K

Related Experiment Videos

Last Updated: Mar 2, 2026

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay
07:21

Fibroblast-Derived 3D Matrix System Applicable to Endothelial Tube Formation Assay

Published on: December 26, 2019

8.0K
Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva
08:43

Three-dimensional Inflammatory Human Tissue Equivalents of Gingiva

Published on: April 3, 2018

10.4K
Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis
07:26

Isolation of Whole Cell Protein Lysates from Mouse Facial Processes and Cultured Palatal Mesenchyme Cells for Phosphoprotein Analysis

Published on: April 1, 2022

2.5K

Area of Science:

  • Cell Biology
  • Biochemistry
  • Dental Research

Background:

  • Eph receptors and ephrins are key in cell signaling and tissue development.
  • The ephrinB1-EphB2 interaction influences odontogenic differentiation of dental pulp cells.
  • Tertiary dentin formation is a critical response to dental injury.

Purpose of the Study:

  • To elucidate the molecular mechanisms of the EphB2/ephrinB1 system in tertiary dentin formation.
  • To investigate the role of insulin-like growth factor 1 (IGF-1) in this process.
  • To evaluate the therapeutic potential of targeting ephrinB1 for pulp regeneration.

Main Methods:

  • Analyzing ephrinB1 and EphB2 expression in developing and injured teeth.
  • Utilizing in vitro studies with dental pulp cells (DPCs) treated with calcium hydroxide (CH) or mineral trioxide aggregate (MTA).
  • Employing pathway inhibitors (Ras/Raf-1/MAPK, PI3K/Akt/mTOR) and gene ablation models (IGF-1 receptor).

Main Results:

  • EphrinB1 and EphB2 show dynamic expression patterns during tooth development and post-injury.
  • IGF-1 expression increased in DPCs treated with CH or MTA.
  • Inhibition of specific signaling pathways affected EphB2 and ephrinB1 expression.
  • IGF-1 receptor ablation in odontoblasts reduced tertiary dentin volume, mineral density, and ephrinB1 expression post-injury.

Conclusions:

  • The IGF-1/ephrinB1 axis is vital for early-stage tertiary dentin formation following tooth injury.
  • This axis regulates key aspects of dentin repair, including volume, mineral density, and odontoblast expression.
  • Targeting ephrinB1 presents a potential avenue for future regenerative pulp therapies.