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Updated: Mar 2, 2026

Pharmacologic Induction of Epidermal Melanin and Protection Against Sunburn in a Humanized Mouse Model
Published on: September 7, 2013
Cyclic AMP-Epac signaling pathway contributes to repression of PUMA transcription in melanoma cells
Alexander J Lakhter1, Samisubbu R Naidu
1Department of Microbiology and Immunology, Indiana University School of Medicine, Indianapolis, Indiana, USA.
Abstract:
The universal second messenger cAMP regulates numerous cellular processes. Although the cAMP-signaling pathway leads to induction of gene transcription, it remains unknown whether this pathway contributes toward suppression of transcription. Here, we show that blockade of cAMP signaling using MDL12330A led to an increase in PUMA transcript levels, but not p21 in melanoma cells. cAMP downstream component Epac activation was essential for suppression of PUMA transcription as an Epac agonist reversed the effects of MDL12330A. These results suggest that transcriptional repression is one of the functions of the cAMP-Epac signaling pathway.
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