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MicroRNA-499-5p regulates porcine myofiber specification by controlling Sox6 expression

X Y Wang1, X L Chen1, Z Q Huang1

  • 11Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,Sichuan 611130,China.

Animal : an International Journal of Animal Bioscience
|May 12, 2017
PubMed

Insights

MicroRNAs (miRNAs) regulate gene expression and are vital for muscle development. This study shows microRNA-499-5p promotes oxidative muscle fiber formation by downregulating Sox6 expression in pigs.

Area of Science:

  • Molecular Biology
  • Genetics
  • Animal Science

Background:

  • MicroRNAs (miRNAs) are small non-coding RNAs regulating gene expression.
  • miRNAs play crucial roles in skeletal muscle development.
  • miRNA-499-5p is inversely related to Sox6, a transcription factor for fast-twitch skeletal muscle.

Purpose of the Study:

  • To investigate the regulatory relationship between miRNA-499-5p and porcine Sox6 (pSox6).
  • To determine the role of miRNA-499-5p in skeletal muscle fiber type determination.

Main Methods:

  • Amplification of porcine Sox6 3'UTR mRNA.
  • Luciferase reporter assay to confirm pSox6 as a miRNA-499-5p target.
  • Analysis of miRNA-499-5p and pSox6 expression in porcine skeletal muscles.
  • Overexpression of miR-499-5p in porcine satellite cells.

Main Results:

  • Verified pSox6 as a direct target of miR-499-5p.
  • Observed inverse correlation between miR-499-5p and pSox6 mRNA levels in pig muscles.
  • Overexpression of miR-499-5p increased MyHC I and MyHC IIa mRNA expression while decreasing pSox6 mRNA.

Conclusions:

  • miR-499-5p downregulates pSox6 expression.
  • miR-499-5p may promote oxidative myofiber formation by targeting pSox6.
Keywords:
miR-499-5poxidative myofibers formationporcine Sox6porcine satellite cellsskeletal muscle

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