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Updated: Mar 2, 2026
Directed Differentiation of Hemogenic Endothelial Cells from Human Pluripotent Stem Cells
Published on: March 31, 2021
[Comparison of differentiated endothelial cells from the embryonic stem cells with human umbilical vein endothelial
Yangui Wang1, Xiaoxiao Mao2, Tianlun Yang2
1Department of Cardiology, Xiangya Hospital, Central South University, Changsha 410008; Department of Geriatrics, Hunan Provincial People's Hospital, Changsha 410005, China.
Objective:
To compare the differentiated endothelial cells from the embryonic stem cells in vitro with human umbilical vein endothelial cells (HUVECs). Methods: Induction of the stem cells HUES9 to endothelial cells follows 2 steps. Stem cells were treated with CHIR99021 (10 µmol/L) and bone morphogenetic protein 4 (25 ng/mL) for 3 days to keep mesoderm state, then subsequent exposure them to VEGF165 (200 ng/mL) and Forskolin (2 µmol/L) to differentiate into endothelial cells. The morphology of differentiated endothelial cells were compared with HUVECs. The surface marker CD144 on differentiated cells and HUVECs were detected. The capabilities of two types of endothelial cells in migration and angiogenesis were examined. Results: The differentiated endothelial cells show the same morphology with HUVECs. After 6 days of differentiation, the efficiency reached 73.4%. The positive percentage of CD144 for the differentiated endothelial cells and HUVECs was 86.6% and 94.4%, respectively. Both of them show capabilities of migration and angiogenesis, especially when they were treated with SB431542 to inhibit TGF-β signal pathway. Conclusion: The method for induction of stem cells to endothelial cells is productivity and it can be used for further study.
