Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Assembly of Complex Microtubule Structures01:32

Assembly of Complex Microtubule Structures

2.7K
Complex microtubule structures are present in resting cells and in dividing cells. In resting cells, they are responsible for maintaining the cellular architecture, tracks for intracellular transport, positioning of organelles, assembly of cilia and flagella. They mediate the bipolar spindle assembly for chromosomal segregation and positioning of the cell division plate in dividing cells. The formation of microtubule complex structures depends on the cell type, cell stage, and cell function.
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cell autonomous inflammation in VEXAS is mediated by cGAS-STING.

bioRxiv : the preprint server for biology·2026
Same author

Impaired Remodeling of Tight Junctions Associated with Loss of Enamel Rod Decussation in Tgfbr2<sup>G357W/+</sup> Mice.

Calcified tissue international·2026
Same author

A distinct vagus-beta cell neural circuit senses glucose and modulates insulin secretion.

Molecular metabolism·2026
Same author

Differential microglial responses to structurally distinct alpha-synuclein polymorphs.

Molecular brain·2025
Same author

Trends of Genitourinary Fistula in Kigali, Rwanda, over a 10-year Period.

International urogynecology journal·2025
Same author

Urge Incontinence After Pelvic Organ Prolapse Surgery: A Secondary Analysis.

Urogynecology (Philadelphia, Pa.)·2025

Related Experiment Video

Updated: Mar 2, 2026

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
09:51

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Published on: December 13, 2017

16.3K

The intestinal tuft cell nanostructure in 3D.

Ben Hoover1, Valentina Baena2, Melanie M Kaelberer3

  • 1School of Medicine, Duke University, Durham, NC, USA.

Scientific Reports
|May 12, 2017
PubMed
Summary
This summary is machine-generated.

Tuft cells possess unique ultrastructures, including cytospinules projecting into neighboring nuclei and a tubular network. These features may facilitate molecular exchange with the gut lumen and adjacent cells.

More Related Videos

Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images
12:45

Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images

Published on: August 31, 2022

3.7K
3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

10.3K

Related Experiment Videos

Last Updated: Mar 2, 2026

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
09:51

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids

Published on: December 13, 2017

16.3K
Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images
12:45

Detection and Quantification of Tunneling Nanotubes Using 3D Volume View Images

Published on: August 31, 2022

3.7K
3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography
07:01

3D Imaging of Soft-Tissue Samples using an X-ray Specific Staining Method and Nanoscopic Computed Tomography

Published on: October 24, 2019

10.3K

Area of Science:

  • Cell Biology
  • Gastroenterology
  • Microscopy

Background:

  • Tuft cells, a type of intestinal epithelial cell, have historically been considered enigmatic due to their peculiar morphology.
  • Understanding their ultrastructure is crucial for elucidating their function.

Purpose of the Study:

  • To provide a detailed volumetric ultrastructure of intestinal tuft cells at nanometer resolution.
  • To identify novel cellular components and their potential roles.

Main Methods:

  • Serial Block-Face (SBF) Scanning Electron Microscopy (SEM)
  • Automated Tape-collecting Ultra-Microtome (ATUM) SEM
  • Volume rendering of serial sections

Main Results:

  • Identified a short basal cytoplasmic process lacking secretory vesicles.
  • Discovered thin cytospinules extending from tuft cells into neighboring epithelial cell nuclei.
  • Revealed an interconnected tubular network within tuft cells, linking microvilli to the rough endoplasmic reticulum.

Conclusions:

  • The unique ultrastructure, including cytospinules and the tubular network, suggests a role for tuft cells in molecular exchange.
  • These structures may facilitate communication with neighboring cell nuclei and the gut lumen.