Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Effects of EDTA on End-Point Detection Methods

Effects of EDTA on End-Point Detection Methods

Different methods, such as visual observance of metal-ion indicators, spectroscopic techniques, and potentiometric methods, can determine the endpoint of an EDTA titration.
In the visual method, metal-ion indicators (metallochromic dyes), which have distinct colors in their free and complex forms, are added to the mixture to signal the titration's end point. They form stable complexes with metal ions, but these complexes are weaker than the corresponding metal–EDTA complexes. As a...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mid-term outcomes of stemless local bone autografting for moderate defects in primary total knee arthroplasty: a series of one hundred and twenty five cases.

International orthopaedics·2026

Same author

The Efficacy and Safety of Nemolizumab in Patients with Prurigo Nodularis: A Systematic Review and Meta-Analysis of Randomized Controlled Trials.

Dermatology practical & conceptual·2025

Same author

Systematic Review and Meta-Analysis of the Efficacy and Risk of Adverse Events of Dupilumab in the Treatment of Prurigo Nodularis.

The Australasian journal of dermatology·2025

Same author

Sweet syndrome in patients with and without malignancy: a retrospective study of 66 cases from a tertiary care centre.

European journal of dermatology : EJD·2024

Same author

Assessment, Prevalence, and Correlates of Frailty among Moroccan People Aged 50 and above Living with HIV.

International journal of mycobacteriology·2024

Same author

Hepatic Metastasis Revealing a Melanoma of the Penis: Case Report.

JMIR dermatology·2023

Search research articles

Related Experiment Video

Updated: Mar 2, 2026

Microfluidics in Assessing Platelet Function

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

[A non-EDTA-dependent pseudothrombocytopenia: about a case].

Hicham Titou¹, Youssef Jalal², Mohammed Boui¹

¹Service de Dermatologie Vénérologie, Hôpital Militaire d'Instruction Mohamed V, Faculté de Médecine de Rabat, Maroc.

The Pan African Medical Journal

|May 12, 2017

Summary

Pseudothrombocytopenia, a lab artifact from platelet clumping, can mimic serious conditions. Microscopic review is crucial to distinguish it from heparin-induced thrombocytopenia, preventing misdiagnosis.

Keywords:

Anticoagulant heparin platelets pseudothrombocytopenia

More Related Videos

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

Related Experiment Videos

Last Updated: Mar 2, 2026

Microfluidics in Assessing Platelet Function

Microfluidics in Assessing Platelet Function

Published on: November 8, 2024

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Procoagulant Platelet Characterization by Measuring Phosphatidylserine Exposure and Microvesicle Release from Human Purified Platelets

Published on: November 29, 2024

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Megakaryocyte Differentiation and Platelet Formation from Human Cord Blood-derived CD34+ Cells

Published on: December 27, 2017

Area of Science:

Hematology
Laboratory Medicine
Clinical Pathology

Background:

Pseudothrombocytopenia is an uncommon laboratory finding characterized by the in vitro agglutination of platelets.
It can lead to diagnostic challenges, particularly when occurring in patients treated with heparin.
Differentiating pseudothrombocytopenia from heparin-induced thrombocytopenia is critical for appropriate patient management.

Observation:

Microscopic examination of peripheral blood smears is essential for identifying platelet clumping.
This direct visualization confirms the artefactual nature of low platelet counts in pseudothrombocytopenia.
The presence of platelet aggregates on smear is the hallmark of this condition.

Findings:

The study aimed to clarify the distinction between pseudothrombocytopenia and heparin-induced thrombocytopenia.
In vitro platelet agglutination is the cause of pseudothrombocytopenia, a non-pathological phenomenon.
Accurate diagnosis relies on careful laboratory assessment, primarily peripheral blood smear review.

Implications:

Correctly identifying pseudothrombocytopenia prevents unnecessary patient anxiety and costly, potentially harmful interventions.
Distinguishing it from heparin-induced thrombocytopenia avoids inappropriate treatment cessation or initiation.
Emphasizes the continued importance of manual microscopy in hematological diagnostics.