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Updated: Mar 2, 2026

Author Spotlight: Detecting Low-Abundant Host Cell Proteins in Drug Products Using Enrichment Beads and Limited Digestion
Published on: January 19, 2024
Characterization of Host Cell Proteins (HCPs) in CHO Cell Bioprocesses
Catherine E M Hogwood1, Lesley M Chiverton1, C Mark Smales2
1Industrial Biotechnology Centre and School of Biosciences, University of Kent, Canterbury, Kent, UK.
Abstract:
Host cell protein content during bioprocessing of biotherapeutic proteins generated from cultured Chinese hamster ovary (CHO) cells is typically measured using immunological and gel-based methods. Estimation of HCP concentration is usually undertaken using Enzyme-Linked ImmunoSorbent Assays (ELISA), while estimation of HCP clearance/presence can be achieved by comparing 2D-PAGE images of samples and by undertaking western blotting of 2D-PAGE analyzed samples. Here, we describe the analyses of HCP content using these methodologies.
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