Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Recycling Endosomes and Transcytosis00:58

Recycling Endosomes and Transcytosis

3.7K
The recycling endosome, also known as the endosomal recycling compartment (ERC), is a part of the slow-recycling process of the endocytic pathway. Molecules internalized through receptor-mediated endocytosis are either degraded in the lysosomes or are recycled to the plasma membrane through the fast- or slow-recycling route.
The recycling endosome is not a single organelle but an extensively tubulated network of recycling pathways. It functions in storing molecules or transporting them across...
3.7K
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

3.4K
Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
3.4K
Cotranslational Protein Translocation01:20

Cotranslational Protein Translocation

10.7K
Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
Sec61 channel partners for cotranslational translocation
During cotranslational translocation, the Sec61 channel partners with the signal recognition particle (SRP), the signal recognition particle receptor (SR), and the ribosomes to transport the nascent polypeptide chain...
10.7K
Intralumenal Vesicles and Multivesicular Bodies01:38

Intralumenal Vesicles and Multivesicular Bodies

5.0K
Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
5.0K
Bacterial Translocation and Protein Secretion01:26

Bacterial Translocation and Protein Secretion

853
Bacterial protein secretion involves translocation systems to ensure proteins reach their designated locations, including the plasma membrane, periplasm, outer membrane, or the external environment. These translocation systems are vital for bacterial physiology, supporting processes like membrane assembly, enzymatic activity in the periplasm, and interactions with the external environment. The division of labor between Sec and Tat pathways ensures efficiency in handling proteins with diverse...
853
Intracellular Movement of Viruses and Bacteria01:10

Intracellular Movement of Viruses and Bacteria

3.7K
Intracellular bacteria and viruses often comprise a group of highly infectious pathogens that can cause several diseases. Bacterial pathogens include those belonging to the genus Rickettsia responsible for conditions such as rocky mountain spotted fever and the Mediterranean spotted fever; Chlamydia, a genus responsible for a sexually transmitted disease; Coxiella burnetii, an agent responsible for Q fever. Viral pathogens include vaccinia—a poxvirus, and herpes simplex virus—a...
3.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Correction to: Engineered Alzheimer Organoids Validate the Link Between Intracellular and Soluble p-Tau Biomarkers and Highlight the Contribution of Astrocytic Tau.

Neuroscience bulletin·2026
Same author

A spatiotemporal gating hypothesis for psilocybin plasticity: reconciling the 5-HT₂A-TrkB mechanistic paradox.

Cell discovery·2026
Same author

4D single-cell spatial transcriptomics reveals dynamic morphogenetic gradients and regenerative domains in planarians.

GigaScience·2026
Same author

Direct Generation of Human Neuronal Cells from Adult Astrocytes by Small Molecules.

Stem cell reports·2026
Same author

Head-to-head comparison of nuclear imaging approaches to quantify tumor CD8<sup>+</sup> T-cell infiltration.

Immunotherapy advances·2026
Same author

Multimodal clocks of human aging.

Cell·2026

Related Experiment Video

Updated: Mar 2, 2026

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1
07:42

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

977

A Rab20-Dependent Membrane Trafficking Pathway Controls M. tuberculosis Replication by Regulating Phagosome

Laura Schnettger1, Angela Rodgers2, Urska Repnik3

  • 1Host-Pathogen Interactions In Tuberculosis Laboratory, The Francis Crick Institute, 1 Midland Road, London NW1 1AT, UK.

Cell Host & Microbe
|May 12, 2017
PubMed
Summary

Researchers discovered a new immune defense pathway in macrophages that keeps Mycobacterium tuberculosis (Mtb) contained within phagolysosomes, crucial for controlling bacterial infection and replication.

Keywords:
Rab GTPasesRab20macrophagemycobacteriumphagosometuberculosis

More Related Videos

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

8.2K
Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

1.3K

Related Experiment Videos

Last Updated: Mar 2, 2026

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1
07:42

Fluorescence Assays for the Study of Mycobacterium tuberculosis Interaction with the Immune Receptor SLAMF1

Published on: February 28, 2025

977
A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening
15:28

A Microscopic Phenotypic Assay for the Quantification of Intracellular Mycobacteria Adapted for High-throughput/High-content Screening

Published on: January 17, 2014

8.2K
Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis
07:32

Preparation of Mycobacterium Tuberculosis Culture Filtrate to Understand TB Pathogenesis

Published on: March 28, 2025

1.3K

Area of Science:

  • Cellular microbiology
  • Immunology
  • Pathogen-host interactions

Background:

  • Mycobacterium tuberculosis (Mtb) is an intracellular pathogen that resides within phagosomes.
  • Mtb can disrupt phagosomes to access the host cell cytosol.
  • Host mechanisms maintaining Mtb phagosome integrity are largely unknown.

Purpose of the Study:

  • To investigate host pathways that maintain the integrity of Mtb-containing phagosomes.
  • To identify mechanisms controlling Mtb's intracellular lifestyle.

Main Methods:

  • Examined spatiotemporal dynamics of Mtb-containing phagosomes in macrophages.
  • Identified an interferon-gamma-stimulated and Rab20-dependent membrane trafficking pathway.

Main Results:

  • This pathway promotes endosomal membrane influx, maintaining phagosome integrity.
  • It is essential for controlling Mtb replication within macrophages.
  • Rab20 is significantly upregulated in sputum from active tuberculosis patients.

Conclusions:

  • Uncovered an immune-regulated cellular defense pathway.
  • This pathway confines Mtb within intact membrane-bound compartments for elimination.
  • Rab20 plays a critical role in host defense against Mtb.