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Related Experiment Video

Updated: Mar 2, 2026

An Analytical Tool-box for Comprehensive Biochemical, Structural and Transcriptome Evaluation of Oral Biofilms Mediated by Mutans Streptococci
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Streptococcus mutans membrane lipid composition: Virulence factors and structural parameters.

M A Bojanich1, R O Calderón2

  • 1Department of Biological Chemistry, Oral Biology Department, School of Dentistry, National University of Córdoba, Argentina.

Archives of Oral Biology
|May 12, 2017
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Summary

Dental caries location impacts Streptococcus mutans membrane fatty acids, enhancing acid survival and virulence in acidic environments. This study reveals how biofilm location affects bacterial adaptation to oral acidity.

Keywords:
ATPase activityFatty acidsOral microorganisms

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Area of Science:

  • Microbiology
  • Oral Health
  • Biochemistry

Background:

  • Streptococcus mutans is a key pathogen in dental caries.
  • Dental biofilm composition varies by tooth surface and caries status.
  • Bacterial membrane properties influence virulence and survival.

Purpose of the Study:

  • To investigate the association between dental biofilm location and Streptococcus mutans membrane fatty acid profiles.
  • To determine if these shifts affect bacterial virulence factors.
  • To understand the impact of environmental pH on S. mutans adaptation.

Main Methods:

  • S. mutans strains were isolated from dental biofilm on sound and carious smooth and occlusal surfaces.
  • Lipid composition, membrane structure, acid survival, and ATPase activity were analyzed.
  • Experiments were conducted at pH 7 and pH 5.

Main Results:

  • At pH 5, strains from carious smooth surfaces showed increased unsaturated and long-chain fatty acids.
  • Proton ATPase activity increased in strains from carious smooth surfaces at pH 5.
  • Changes correlated with membrane organization and increased acyl chain interaction.

Conclusions:

  • The acidic environment of smooth surface caries alters S. mutans membrane organization and lipid profiles.
  • These adaptations enhance ATPase activity and acid survival.
  • Biofilm location significantly influences S. mutans virulence factor expression and adaptation strategies.