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Related Concept Videos

MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
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MicroRNAs01:22

MicroRNAs

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MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
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Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

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In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
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Pre-mRNA Processing: Modification of pre-mRNA Ends01:35

Pre-mRNA Processing: Modification of pre-mRNA Ends

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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a cap to the 5' end of the growing transcript. In this process, a 5' phosphate is replaced by modified guanosine that has a methyl group attached (7-methyl guanosine). This 5' cap helps...
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RNA Stability01:53

RNA Stability

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Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
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mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

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The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
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Related Experiment Video

Updated: Mar 2, 2026

Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis
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Methylated RNA Immunoprecipitation Assay to Study m5C Modification in Arabidopsis

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m6A Modification and Implications for microRNAs.

Ayse Elif Erson-Bensan1, Oguzhan Begik1

  • 1Department of Biological Sciences, Middle East Technical University (METU), Ankara, 06800. Turkey.

Microrna (Shariqah, United Arab Emirates)
|May 13, 2017
PubMed
Summary
This summary is machine-generated.

Methylation of Adenine (m6A) is a key RNA modification impacting gene regulation. This review explores m6A

Keywords:
3'UTRRNA methylationm6AmRNAmicroRNAtranscriptome

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Characterizing RNA Modifications in Single Neurons Using Mass Spectrometry
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Area of Science:

  • Molecular Biology
  • Epigenetics
  • Gene Regulation

Background:

  • Over 100 chemical modifications exist for RNA.
  • RNA methylation, specifically N6-methyladenosine (m6A), is a prevalent and extensively studied modification.
  • m6A is implicated in various biological processes.

Purpose of the Study:

  • To review recent literature on m6A modification.
  • To explore the implications of m6A in microRNA (miRNA)-mediated gene expression regulation.
  • To elucidate the roles of m6A in miRNA biogenesis, mRNA-miRNA interactions, and m6A target selection.

Main Methods:

  • Literature review of recent studies on m6A modification.
  • Analysis of m6A's role in miRNA biogenesis.
  • Investigation of m6A's influence on mRNA-miRNA interactions and target selection.

Main Results:

  • m6A modification is widespread across the transcriptome.
  • m6A plays a significant role in regulating gene expression through miRNA pathways.
  • Specific implications include effects on miRNA biogenesis, mRNA-miRNA binding, and m6A target recognition.

Conclusions:

  • Understanding m6A is crucial for deciphering complex transcriptome regulation.
  • m6A's role in gene regulation is significant in both normal physiological and disease states.
  • Further research into m6A functions will enhance our knowledge of cellular processes.