Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Plasminogen Recruitment by <i>Staphylococcus aureus</i> SdrC Reveals a Tractable Antivirulence Target.

ACS infectious diseases·2026
Same author

Genetic determinants of Staphylococcus aureus adhesion shape virulence trade-offs in bacteremia.

Nature communications·2026
Same author

Nanoscale Mechanics of FnBPB-Mediated Adhesion of <i>Staphylococcus aureus</i> to Skin Ligands.

ACS nano·2026
Same author

Large-scale characterisation of the nasal microbiome redefines Staphylococcus aureus colonisation status.

Nature communications·2025
Same author

Ultrastrong <i>Staphylococcus aureus</i> adhesion to human skin: Calcium as a key regulator of noncovalent interactions.

Science advances·2025
Same author

Force Nanoscopy Demonstrates Stress-Activated Adhesion between <i>Staphylococcus aureus</i> Iron-Regulated Surface Determinant Protein B and Host Toll-like Receptor 4.

ACS nano·2025

Related Experiment Video

Updated: Mar 2, 2026

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
09:14

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM

Published on: October 2, 2012

10.6K

Live-Cell Nanoscopy in Antiadhesion Therapy.

Joan A Geoghegan1, Timothy J Foster1, Pietro Speziale2

  • 1Department of Microbiology, Moyne Institute of Preventive Medicine, School of Genetics and Microbiology, Trinity College Dublin, Dublin 2, Ireland.

Trends in Microbiology
|May 13, 2017
PubMed
Summary

Live-cell nanoscopy effectively evaluated anti-adhesion therapies against uropathogenic Escherichia coli and Staphylococcus aureus. This advanced imaging technology shows promise for developing new treatments against bacterial pathogens.

Keywords:
Staphylococcus aureusantiadhesion therapybacterial adhesionbiofilmsnanoscopy

More Related Videos

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions
10:50

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions

Published on: April 3, 2014

10.9K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.3K

Related Experiment Videos

Last Updated: Mar 2, 2026

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM
09:14

Nanotopology of Cell Adhesion upon Variable-Angle Total Internal Reflection Fluorescence Microscopy VA-TIRFM

Published on: October 2, 2012

10.6K
Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions
10:50

Nanopodia - Thin, Fragile Membrane Projections with Roles in Cell Movement and Intercellular Interactions

Published on: April 3, 2014

10.9K
Ligand Nano-cluster Arrays in a Supported Lipid Bilayer
10:34

Ligand Nano-cluster Arrays in a Supported Lipid Bilayer

Published on: April 23, 2017

7.3K

Area of Science:

  • Microscopy and imaging technologies
  • Bacterial pathogenesis and host-cell interactions
  • Drug discovery and development

Background:

  • Bacterial adhesion is a critical factor in pathogenesis for pathogens like uropathogenic Escherichia coli (UPEC) and Staphylococcus aureus.
  • Developing effective anti-adhesion therapies is crucial for combating bacterial infections.
  • Live-cell nanoscopy offers advanced capabilities for studying dynamic cellular processes in real-time.

Purpose of the Study:

  • To assess the efficacy of glycoconjugates and monoclonal antibodies in inhibiting UPEC and S. aureus adhesion, respectively.
  • To evaluate the impact of a synthetic peptide on S. aureus surface attachment and cell-cell aggregation.
  • To demonstrate the utility of live-cell nanoscopy in the study of anti-adhesion strategies.

Main Methods:

  • Live-cell nanoscopy was employed to visualize and quantify bacterial adhesion and interaction dynamics.
  • Specific inhibitors, including glycoconjugates, monoclonal antibodies, and a synthetic peptide, were tested against bacterial pathogens.
  • Quantitative analysis of bacterial surface attachment and cell-cell association was performed using high-resolution imaging.

Main Results:

  • Live-cell nanoscopy successfully assessed the inhibition of UPEC adhesion by glycoconjugates.
  • The study confirmed the inhibition of S. aureus adhesion and cell-cell association by monoclonal antibodies and a synthetic peptide.
  • Nanoscopy provided detailed insights into the mechanisms of adhesion inhibition at the nanoscale.

Conclusions:

  • Live-cell nanoscopy is a powerful tool for evaluating anti-adhesion strategies against bacterial pathogens.
  • The findings support the potential of glycoconjugates, antibodies, and peptides as therapeutic agents.
  • This technology holds significant promise for the future development of anti-adhesion therapies.