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DNA damage by chemically generated singlet oxygen.

M V Lafleur1, A W Nieuwint, J M Aubry

  • 1Department of Biophysics, Free University, Amsterdam, The Netherlands.

Free Radical Research Communications
|January 1, 1987
PubMed
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Singlet oxygen (1O2) does not cause DNA interstrand crosslinking or direct strand breaks. However, 1O2 exposure inactivates DNA, suggesting damage to DNA bases or sugars, particularly in single-stranded DNA.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Oxidative Stress

Background:

  • Reactive oxygen species, including singlet oxygen (1O2), play roles in cellular processes and damage.
  • Understanding DNA interactions with 1O2 is crucial for assessing oxidative damage mechanisms.

Purpose of the Study:

  • To investigate the interactions between singlet oxygen (1O2) and DNA.
  • To determine if 1O2 induces DNA interstrand crosslinking or strand breaks.
  • To assess the biological consequences of 1O2 exposure on DNA infectivity.

Main Methods:

  • A naphthalenic endoperoxide was used as a non-photochemical source of 1O2.
  • Hydroxyl apatite chromatography was used to analyze DNA interstrand crosslinking.
  • Sucrose gradient centrifugation analyzed DNA strand breaks in bacteriophage phi X174 DNA.

Related Experiment Videos

  • Plaque-forming assays assessed the infectivity of treated DNA in Escherichia coli spheroplasts.

    • Main Results:

    • No evidence of 1O2-induced interstrand crosslinking in high molecular weight DNA.
    • No direct strand breaks were detected in single-stranded (ss) or double-stranded (ds) DNA at neutral pH.
    • Extensive strand breakage occurred in ss and ds DNA after alkaline treatment.
    • 1O2 exposure caused time- and dose-dependent inactivation of DNA infectivity, with ss DNA being more sensitive.

    Conclusions:

    • 1O2 does not induce DNA interstrand crosslinking or direct backbone breakage.
    • The observed DNA inactivation is likely due to damage to the DNA base or sugar moiety.
    • Single-stranded DNA is more susceptible to 1O2-induced inactivation than double-stranded DNA.