Quantitative analysis of amphiphilic N-alkyloxypyridinecarboximidamide by liquid chromatography-tandem mass
Irmina Wojciechowska1, Aleksandra Wojciechowska1, Karolina Wieszczycka1
1Institute of Chemical Technology and Engineering, Poznan University of Technology, Berdychowo St. 4, 60-965 Poznan, Poland.
Abstract:
LC-MS/MS method to determine hydrophobic N-alkyloxy substituted amidines: N-(2-ethylhexyloxy)pyridine-2-carboximidamide, N-(2-ethylhexyloxy)pyridine-3-carboximidamide, N-(2-ethylhexyloxy)pyridine-4-carboximidamide, N-decyloxy pyridine-2-carboximidamide, N-decyloxypyridine-3-carboximidamide and N-decyloxypyridine-4-carboximidamide was developed and validated in terms of linearity, precision and accuracy. The developed method was successfully applied to monitor and control the synthesis process. The experimental data points indicated that the straight chain alkyl bromide reacted most rapidly than branched alkyl bromide and the enhancement of the reaction efficiency strongly depended on reaction temperature.
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