Cadaver embalming fluid for surgical training courses: modified Larssen solution.

Area of Science:

• Anatomical studies
• Medical education
• Embalming techniques

Background:

• Traditional 10% formalin (F10) fixation presents drawbacks including odor, irritation, discoloration, and rigidity.
• Need for improved cadaver preservation methods for anatomical training.

Purpose of the Study:

• To evaluate modified Larssen solution (MLS) as a simple, cost-effective embalming agent.
• To assess MLS for long-term preservation of tissue color, texture, pliability, and flexibility.
• To determine suitability for repeated surgical training without odor or irritation.

Main Methods:

• Human cadavers (8 male, 4 female) preserved using modified Larssen solution (MLS).
• Stored at -18/-20°C, thawed 3 days prior to use.
• Used in postgraduate hands-on courses, remaining at room temperature during training sessions.
• Organoleptic comparison with fresh frozen (FF) and F10-fixed cadavers.

Main Results:

• MLS-preserved cadavers exhibited life-like tissue colors (muscles, fasciae, fatty tissue, nerves, vessels).
• No significant odor or signs of putrefaction were observed in MLS cadavers.
• Positive feedback from 30 trainers and 252 trainees on tissue quality.

Conclusions:

• Modified Larssen solution (MLS) is a sustainable and affordable soft cadaver embalming method.
• MLS application is straightforward, requiring no specialized equipment.
• Demonstrates success in preserving human cadavers for educational purposes.