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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Genome characterization of sweet potato symptomless virus 1: a mastrevirus with an unusual nonanucleotide sequence
Mengji Cao1,2, Pingxiu Lan1,3, Fan Li3
1National Citrus Engineering Research Center, Citrus Research Institute, Southwest University, Chongqing, 400712, China.
Abstract:
Complete genomic sequences of nine isolates of sweet potato symptomless virus 1 (SPSMV-1), a virus of the genus Mastrevirus in the family Geminiviridae, were determined from sweet potato accessions from different countries and found to be 2,559-2,602 nucleotides in length. These isolates shared 97-100% genome sequence identity and had an unusual nonanucleotide sequence (TAAGATTCC) in a large intergenic region as well as an additional open reading frame, C3, which is conserved in dicot-infecting mastreviruses.
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