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Highly complex substrates lead to dynamic bacterial community for polyhydroxyalkanoates production.

Diogo Queirós1, Alexandre Fonseca1, Simona Rossetti2

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Mixed microbial cultures (MMC) offer a cost-effective method for producing polyhydroxyalkanoates (PHA). Long-term reactor operation demonstrated stable PHA production despite a dynamic microbial community, highlighting the potential for industrial application.

Keywords:
Bacterial community dynamicsHardwood spent sulfite liquor (HSSL)Mixed microbial cultures (MMC)Molecular methodsPolyhydroxyalkanoates (PHA)

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Area of Science:

  • Biotechnology
  • Microbial Ecology
  • Bioreactor Engineering

Background:

  • Polyhydroxyalkanoates (PHA) are biodegradable polymers with significant industrial potential.
  • Reducing PHA production costs is crucial for widespread commercialization.
  • Mixed microbial cultures (MMC) and waste substrates offer a promising approach to lower production expenses.

Purpose of the Study:

  • To assess the long-term stability of a sequencing batch reactor (SBR) using MMC for PHA production.
  • To investigate the dynamics of the microbial community structure in relation to reactor performance.
  • To evaluate the feasibility of industrial PHA production using cost-effective strategies.

Main Methods:

  • Long-term operation of a sequencing batch reactor (SBR) under feast/famine conditions.
  • Microbial community analysis using Fluorescence In Situ Hybridization (FISH) and Denaturing Gradient Gel Electrophoresis (DGGE).
  • 16S rRNA gene clone library sequencing for detailed microbial identification.

Main Results:

  • The microbial community within the SBR was highly dynamic but dominated by PHA-storing Alphaproteobacteria (e.g., Paracoccus, Rhodobacter).
  • Non-PHA accumulating bacteria (e.g., Agrobacterium, Flavobacteria, Brachymonas) also thrived within the community.
  • Despite community fluctuations, polyhydroxyalkanoates (PHA) storage performance remained stable throughout the operational period.

Conclusions:

  • Reactor stability in terms of polyhydroxyalkanoates (PHA) production can be maintained with mixed microbial cultures (MMC) even with significant microbial community shifts.
  • Understanding the relationship between microbial community structure and process operation is key for optimizing bioreactor performance.
  • This study supports the industrial viability of using MMC and waste substrates for cost-effective PHA manufacturing.