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Related Concept Videos

Allergic Reactions: Anaphylaxis01:30

Allergic Reactions: Anaphylaxis

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Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin,...
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A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
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Related Experiment Video

Updated: Mar 2, 2026

Basophil Activation Test for Allergy Diagnosis
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In Vitro Diagnostic Testing for Antibiotic Allergy.

Inmaculada Doña1, Maria J Torres2,3, Maria I Montañez3,4

  • 1Allergy Unit, IBIMA-Regional University Hospital of Malaga-UMA, Málaga, Spain.

Allergy, Asthma & Immunology Research
|May 13, 2017
PubMed
Summary

Antibiotic allergy affects up to 10% of people globally. This review explores in vitro diagnostic tests, like immunoassays and basophil activation tests, for accurately diagnosing antibiotic allergies, especially when in vivo tests are unsuitable.

Keywords:
Antibioticdrug allergyin vitro test

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Area of Science:

  • Immunology
  • Pharmacology
  • Clinical Medicine

Background:

  • Antibiotic allergy is a significant global health concern, affecting up to 10% of the population.
  • Prevalence of reactions varies among antibiotic classes, with beta-lactams being most frequently reported.
  • Current diagnostic methods, including clinical history and in vivo tests, have limitations in accuracy and applicability.

Purpose of the Study:

  • To review and describe various in vitro techniques for diagnosing antibiotic allergy.
  • To highlight the utility of in vitro tests in overcoming limitations of traditional diagnostic methods.

Main Methods:

  • Review of current literature on in vitro diagnostic techniques for antibiotic allergy.
  • Focus on immunoassays, basophil activation tests (for immediate reactions), and lymphocyte transformation tests (for non-immediate reactions).

Main Results:

  • In vitro tests offer a valuable alternative or adjunct to in vivo testing, particularly for severe reactions or drugs with high false-positive rates.
  • Immunoassays and basophil activation tests are key for immediate hypersensitivity.
  • Lymphocyte transformation tests are more suitable for diagnosing delayed reactions.

Conclusions:

  • In vitro diagnostic methods provide crucial tools for identifying antibiotic allergies, complementing traditional approaches.
  • These tests are essential for accurate diagnosis and management of antibiotic hypersensitivity, improving patient safety and treatment outcomes.