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Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Epigenetic Regulation

Epigenetic Regulation

Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.

Epigenetic Regulation

Epigenetic Regulation

Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...

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Related Experiment Video

Updated: Jun 26, 2026

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

A Semiautomated ChIP-Seq Procedure for Large-scale Epigenetic Studies

Published on: August 13, 2020

Epimetheus - a multi-profile normalizer for epigenomic sequencing data.

Mohamed-Ashick M Saleem¹, Marco-Antonio Mendoza-Parra^2,3,4,5, Pierre-Etienne Cholley¹

¹Equipe Labellisée Ligue Contre le Cancer, Department of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire, Illkirch, France.

BMC Bioinformatics

|May 14, 2017

Summary

This summary is machine-generated.

Epimetheus normalizes epigenomic data, addressing variability in Chromatin ImmunoPrecipitation-Sequencing (ChIP-Seq) assays. This tool enables accurate comparative studies of gene regulation across diverse biological conditions.

Keywords:

ChIP-seq Epigenome Multi-sample analysis Quantile-based data normalization

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Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry

Extraction of Histones from Clinical Specimens for Epigenetic Profiling by Mass Spectrometry

Published on: November 21, 2025

Area of Science:

Epigenomics
Genomics
Computational Biology

Background:

Vast amounts of next-generation sequencing (NGS)-based epigenomic data are publicly available, offering significant potential for integrative and comparative studies of gene regulatory mechanisms.
Identifying functional patterns of chromatin states is crucial for understanding the epigenome's role in normal physiology and disease, but assay variability, particularly in Chromatin ImmunoPrecipitation-Sequencing (ChIP-Seq), presents a major challenge.
Standard data normalization methods for ChIP-Seq data, such as linear scaling, can introduce biases or are limited to specific genomic regions.

Purpose of the Study:

To develop a novel, genome-wide normalization tool for histone modification and related epigenomic datasets that overcomes the limitations of existing methods.
To provide a robust solution for addressing sequencing depth variation and assay variability in comparative epigenomic studies without introducing external biases.

Main Methods:

Development of Epimetheus, a genome-wide normalization tool utilizing a quantile-based, multi-profile approach.
Application of the tool to histone modification data and other related epigenomic datasets.

Main Results:

Epimetheus provides genome-wide normalization, overcoming the restrictions of region-specific tools.
The tool effectively addresses biases associated with assay variability in epigenomic data.

Conclusions:

Epimetheus has been successfully applied in prior studies, including those on X inactivation in breast cancer and integrative analyses of neuronal cell fate and tumorigenesis.
The Epimetheus tool is freely accessible to the scientific community, facilitating advanced epigenomic research.