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Effect of some aldoses on growth of Saccharomyces cerevisiae inhibited with molybdenum
Abstract:
The inhibitory effect of molybdenum ions on growth of yeasts at pH 5.5 was found to be decreased by aldoses in the following order: D-talose greater than L-mannose greater than L-ribose greater than D-lyxose greater than L-galactose greater than L-arabinose greater than L-glucose greater than L-xylose. Increased concentrations of molybdenum brought about morphological changes of yeast cells. Cells grown under these conditions were smaller, had thicker walls and formed clusters.
Insights
Molybdenum ions inhibit yeast growth, but aldose sugars like D-talose reduce this effect. High molybdenum concentrations also alter yeast cell morphology, causing smaller cells, thicker walls, and clustering.
Area of Science:
- Microbiology
- Biochemistry
- Environmental Science
Background:
- Molybdenum is an essential trace element for many organisms, but its effects on microbial growth can be concentration-dependent.
- Yeast, a common model organism, is susceptible to various environmental stressors, including heavy metal ions.
- Aldoses are simple sugars that can interact with metal ions, potentially altering their bioavailability or toxicity.
Purpose of the Study:
- To investigate the inhibitory effect of molybdenum ions on yeast growth at a specific pH.
- To determine the influence of different aldoses on the toxicity of molybdenum ions.
- To observe morphological changes in yeast cells exposed to varying molybdenum concentrations.
Main Methods:
- Yeast cultures were grown at pH 5.5 in the presence of molybdenum ions and various aldoses.
- The inhibitory effect of molybdenum was assessed by comparing growth rates in the presence and absence of aldoses.
- Yeast cell morphology was examined under microscopy after exposure to increased molybdenum concentrations.
Main Results:
- The inhibitory effect of molybdenum ions on yeast growth was reduced by aldoses in a specific order, with D-talose showing the greatest mitigating effect.
- Increased concentrations of molybdenum ions led to significant morphological alterations in yeast cells.
- Affected yeast cells were smaller, exhibited thicker cell walls, and tended to form clusters.
Conclusions:
- Aldoses can modulate the toxicity of molybdenum ions towards yeast, with varying efficacy among different sugar types.
- Molybdenum ions induce distinct morphological changes in yeast, indicating a stress response.
- Understanding these interactions is crucial for managing microbial processes in molybdenum-containing environments.