Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[A decrease in the stimulated level of free intracellular calcium in thrombocytes during refractoriness].

E Ia Pozin, E G Popov, I Iu Gavrilov

    Biulleten' Eksperimental'Noi Biologii I Meditsiny
    |December 1, 1988
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    The Effects of Hindlimb Unloading of Rats on Rheographic Parameters of Femoral and Brachial Arteries In Situ.

    Bulletin of experimental biology and medicine·2024
    Same author

    Pulsatile Features of Major Arteries in Rats during Hypotension Caused by Acute Blood Loss.

    Bulletin of experimental biology and medicine·2024
    Same author

    [Platelet adhesion mediated by von Willebrand factor in patients with premature coronary artery disease].

    Kardiologiia·2023
    Same author

    Monomeric form of C-reactive protein in the assessment of the residual inflammatory cardiovascular risk in patients with subclinical carotid atherosclerosis.

    Kardiologiia·2022
    Same author

    Complex Interaction of Platelets, von Willebrand Factor and Leukocytes, in Whole Blood at High Shear Rates Is Mediated by Platelet GPIIb/IIIa Receptor.

    Bulletin of experimental biology and medicine·2021
    Same author

    Kinetics of Platelet Adhesion to Protein-Coated Surface in Whole Blood Samples at High Flow Rates.

    Bulletin of experimental biology and medicine·2020

    Adenosine diphosphate (ADP) triggers a rise in intracellular calcium (Ca.f) in platelets, leading to aggregation. Platelet refractoriness reduces this response upon subsequent ADP stimulation, even without external calcium.

    Area of Science:

    • Biochemistry
    • Hematology
    • Cellular Physiology

    Background:

    • Platelet activation is a critical process in hemostasis and thrombosis.
    • Intracellular calcium concentration (Ca.f) plays a pivotal role in platelet function.
    • Adenosine diphosphate (ADP) is a key agonist in platelet activation.

    Purpose of the Study:

    • To investigate the role of free intracellular calcium (Ca.f) in ADP-stimulated human platelets.
    • To characterize the changes in Ca.f during platelet activation and aggregation.
    • To examine the phenomenon of platelet refractoriness in response to repeated ADP stimulation.

    Main Methods:

    • Measurement of free intracellular calcium (Ca.f) using the fluorescent probe indo 1.
    • Utilized a multichannel method for precise Ca.f determination.

    Related Experiment Videos

  • Stimulation of platelets with adenosine diphosphate (ADP) in the presence and absence of extracellular calcium.

    • Main Results:

    • ADP induced a rapid, reversible increase in Ca.f and subsequent platelet aggregation when extracellular calcium was present.
    • A second ADP addition resulted in a diminished Ca.f increase and aggregation, indicating platelet refractoriness.
    • ADP also increased Ca.f without extracellular calcium, and refractoriness was observed in this condition as well.

    Conclusions:

    • Free intracellular calcium mobilization is a primary event following ADP stimulation of platelets.
    • Platelet refractoriness to ADP involves mechanisms that limit intracellular calcium release.
    • Extracellular calcium is important for full ADP-induced platelet aggregation but not for the initial Ca.f increase.