A Color Segmentation-Based Method to Quantify Atherosclerotic Lesion Compositions with Immunostaining

There is an increasing recognition that atherosclerotic lesion composition, rather than size, is the determinant of acute events. Immunostaining is a commonly used method to characterize atherosclerotic lesion compositions. Here, we describe a color segmentation-based approach in HSI (hue, saturation, and intensity) color mode, which minimizes subjectivity and produces accurate and consistent quantifications of atherosclerotic lesion compositions.