Related Experiment Video
Updated: Mar 2, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
Published on: June 28, 2016
pH-Triggered Disaggregation-Induced Emission (DIE) probe for sensoring minor-pH changes in near infrared fluorescence
1Hefei National Laboratory for Physical Sciences at the Microscale, CAS Key Laboratory of Soft Matter Chemistry, iChEM, University of Science and Technology of China, Jinzai Road 96#, Hefei 230026, Anhui, PR China.
Abstract:
A new strategy for pH-responsive near infrared (NIR) cyanine dye, based on the concept of triggered disaggregation-induced emission (DIE), has been developed. Schiff base group was introduced to the molecular scaffold of Cy7, and promoted the aggregation of cyanine in pH7.4 buffer due to the strong π-π interaction. While in an acidic (pH 5.8) environment, the Schiff base group was hydrolyzed by acid and the nanoparticles were disaggregated. The vanishing of the intermolecular aggregation-caused quenching thus induced a fluorescence recovery of 96 folds. Moreover, abundant kinds of Schiff base provide more potential cyanine dyes for pH-responsive ultrahigh NIR fluorescence "Off/On" ratio, which could work as the intelligent bio-imaging probes in near future.
More Related Videos
13:47TIRFM and pH-sensitive GFP-probes to Evaluate Neurotransmitter Vesicle Dynamics in SH-SY5Y Neuroblastoma Cells: Cell Imaging and Data Analysis
Published on: January 29, 2015
06:08Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
Published on: December 27, 2018
Related Concept Videos
Fluorescence and Phosphorescence: Instrumentation
Gas Chromatography: Types of Detectors-II
Photoluminescence: Applications
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...