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Related Experiment Video

Updated: Mar 2, 2026

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

A Rapid and Chemical-free Hemoglobin Assay with Photothermal Angular Light Scattering

Published on: December 7, 2016

A nanocluster-based fluorescent sensor for sensitive hemoglobin detection.

Dongqin Yang¹, Huijie Meng¹, Yifeng Tu¹

¹The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, 199 Ren'ai Road, Industrial Park, Suzhou 215123, China.

|May 15, 2017

Summary

This study presents a novel fluorescence sensor for detecting hemoglobin (Hb). The sensor utilizes gold nanoclusters and shows high sensitivity for Hb quantification in blood samples.

Keywords:

Fluorescence Gold nanoclusters Hemoglobin Hydrogen peroxide Quenching

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Area of Science:

Biomedical Engineering
Analytical Chemistry
Nanotechnology

Background:

Hemoglobin (Hb) detection is crucial for diagnosing various medical conditions.
Existing detection methods may lack sensitivity or require complex procedures.
Development of sensitive and rapid Hb detection tools is essential.

Purpose of the Study:

To develop a novel fluorescence sensor for sensitive and quantitative detection of hemoglobin.
To investigate the mechanism behind the fluorescence quenching enhancement.
To validate the sensor's performance in real blood samples.

Main Methods:

Synthesis of gold nanoclusters using bovine serum albumin.
Investigation of fluorescence quenching by hydrogen peroxide and hemoglobin.
Optimization of reaction conditions for hemoglobin detection.
Application of the sensor for blood sample analysis.

Main Results:

Gold nanoclusters exhibited fluorescence quenching in the presence of hydrogen peroxide and hemoglobin.
A synergistic quenching effect was observed when both were present, attributed to hemoglobin's catalytic generation of hydroxyl radicals.
The sensor demonstrated a linear response to hemoglobin concentration from 1-250 nM.
A low limit of detection (0.36 nM) was achieved for hemoglobin.
The sensor was successfully applied to analyze hemoglobin in blood samples with minimal pretreatment.

Conclusions:

A sensitive and efficient fluorescence sensor for hemoglobin detection was successfully developed.
The sensor leverages the catalytic activity of hemoglobin for enhanced signal generation.
This method offers a promising approach for rapid and accurate hemoglobin quantification in clinical diagnostics.