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Related Experiment Video

Updated: Mar 2, 2026

Laser Capture Microdissection - A Demonstration of the Isolation of Individual Dopamine Neurons and the Entire Ventral Tegmental Area
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Using Laser Capture Microdissection to Isolate Cortical Laminae in Nonhuman Primate Brain.

Brian A Corgiat1, Claudius Mueller2

  • 1Center for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MS1A9, Manassas, VA, 20110, USA. bcorgiat@masonlive.gmu.edu.

Methods in Molecular Biology (Clifton, N.J.)
|May 15, 2017
PubMed
Summary

Laser capture microdissection (LCM) isolates specific cells from tissues for detailed analysis. This infrared capture method enables downstream applications like proteomics discovery and RNA profiling.

Keywords:
BrainCortical layerInfrared laserLaser capture microdissectionMolecular profilingNonhuman primateProtein

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Area of Science:

  • Cellular Biology
  • Microscopy Techniques
  • Proteomics

Background:

  • Heterogeneous tissue samples pose challenges for isolating specific cell populations.
  • Laser capture microdissection (LCM) offers a solution for targeted cell procurement.
  • The infrared capture method is a key technique within LCM.

Purpose of the Study:

  • To detail the infrared capture method of laser capture microdissection.
  • To profile the ArcturusXT™ instrument for isolating specific brain regions.
  • To outline sample preparation for downstream proteomic applications.

Main Methods:

  • Utilizing microscopy for visualization of target cells.
  • Employing infrared laser energy to melt a thermolabile polymer, forming a composite.
  • Isolating specific cortical lamina from nonhuman primate brain tissue.

Main Results:

  • Successful isolation of specific cortical lamina using the ArcturusXT™ instrument.
  • Demonstration of LCM compatibility with various downstream applications.
  • Established sample preparation protocols for proteomic analysis.

Conclusions:

  • Laser capture microdissection (infrared capture method) is effective for isolating specific cell populations.
  • The ArcturusXT™ instrument facilitates precise tissue dissection for research.
  • LCM is a valuable tool for advancing fields like proteomics and transcriptomics.