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Updated: Sep 29, 2025

The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis
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The Fastest Western in Town: A Contemporary Twist on the Classic Western Blot Analysis

Published on: February 5, 2014

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Western Blot Techniques

Brianna Kim1

  • 1Ceres Nanosciences, Inc., 9385 Discovery Blvd, Manassas, VA, 20110, USA. bkim@ceresnano.com.

Methods in Molecular Biology (Clifton, N.J.)
|May 15, 2017
PubMed

The Western blot is an important laboratory technique that allows for specific identification and characterization of proteins. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE)-separated proteins are electophoretically transferred to a polyvinylidene fluoride (PVDF) membrane which is then incubated with specific antibodies, then developed to show the protein of interest. Here, we describe the transfer and detection of Outer surface protein A (OspA), a protein only found on the surface of Borrelia burgdorferi, the bacteria responsible for Lyme disease.

Keywords:
Borrelia burgdorferiElectrophoresisImmunoblotLyme diseaseNanotrap® particlesWestern blot

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