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Related Experiment Video

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Profiling of Pre-micro RNAs and microRNAs using Quantitative Real-time PCR qPCR Arrays
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Reverse Phase Protein Microarrays.

Elisa Baldelli1, Valerie Calvert1, Alex Hodge1

  • 1Center for Applied Proteomics and Molecular Medicine, George Mason University, 10920 George Mason Circle, MS 1A9, Manassas, VA, 20110, USA.

Methods in Molecular Biology (Clifton, N.J.)
|May 15, 2017
PubMed
Summary
This summary is machine-generated.

Proteomics research using reverse-phase protein microarrays (RPPA) offers a sensitive method to analyze cellular proteins. RPPA helps map protein interactions and activation for targeted therapies.

Keywords:
Cell lysatesImmunostainingProteomicsReverse-phase protein microarrayTissue lysates

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Area of Science:

  • Proteomics
  • Cellular Biology
  • Biochemistry

Background:

  • Proteins drive cellular functions, making their network analysis crucial for understanding cell biology.
  • Cellular homeostasis alterations are key in personalized medicine and targeted therapies.
  • Advanced proteomic technologies are needed for clinical sample analysis.

Purpose of the Study:

  • To highlight the utility of reverse-phase protein microarrays (RPPA) in proteomic analysis.
  • To demonstrate RPPA's capability in profiling protein activation and modifications.
  • To emphasize RPPA's role in identifying therapeutic targets.

Main Methods:

  • Utilizing reverse-phase protein microarrays (RPPA), a quantitative, high-throughput immunoassay.
  • Analyzing protein expression and post-translational modifications in various biological samples.
  • Employing RPPA for broad proteomic profiling across numerous samples.

Main Results:

  • RPPA provides sensitive and quantitative protein analyses.
  • RPPA effectively captures protein activation and biochemical reactions like phosphorylation and glycosylation.
  • RPPA enables analysis using limited biological material.

Conclusions:

  • RPPA is a valuable tool for comprehensive protein analysis.
  • RPPA data aids in elucidating functional signaling pathways and protein interactions.
  • RPPA facilitates the identification of critical nodes for targeted therapy development.