Related Experiment Video
Updated: Mar 2, 2026
In Vitro Analysis of Myd88-mediated Cellular Immune Response to West Nile Virus Mutant Strain Infection
Published on: November 27, 2014
Cloning, characterization, and function of MyD88 in silvery pomfret (Pampus argenteus) in response to bacterial
Quanxin Gao1, Fei Yin1, Chenjie Zhang1
1Key Laboratory of Marine and Estuarine Fisheries, Ministry of Agriculture, East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, People's Republic of China.
Abstract:
Myeloid differentiation factor 88 (MyD88) is a key and universal downstream adapter for most Toll-like receptors (TLRs) and plays an important role in both the innate and adaptive immune response. In this study, the full-length cDNA of MyD88 (PAMyD88) from silvery pomfret (Pampus argenteus) was cloned and characterized. The PAMyD88 cDNA is 1545bp in length and contains an 876bp open reading frames (ORF). Multiple sequence alignment and phylogenetic tree analyzes revealed that the amino acid sequence of PAMyd88 was homologous to a variety of previously MyD88 molecules characterized from other species. The quantitative real-time reverse transcription-PCR analysis showed that the PAMyD88 mRNA was broadly expressed in all examined tissues, with higher levels observed in the immune-relevant organs. The results showed a significant up-regulation of the TLR2 and PAMyD88 transcript levels in response to L. plantarum and C. butyricum and a substantial expression level of TLR4 and PAMyD88 induced by V. anguillarum. Additionally, a challenge with V. anguillarum resulted in significant apoptosis, whereas the L. plantarum and C. butyricum induced only low levels of apoptosis. These data provide insight into the roles of PAMyD88 in the TLR signaling pathway in response to probiotic and pathogenic bacteria in silvery pomfrets.
