Related Experiment Video
Updated: Mar 2, 2026
09:30
Bacterial Detection & Identification Using Electrochemical Sensors
Published on: April 23, 2013
29.1K
DNA chip based sensor for amperometric detection of infectious pathogens.
Swati Singh1, Ankur Kaushal2, Shashi Khare3
1CSIR-Institute of Genomics and Integrative Biology, Mall Road, Delhi, 110007, India; Academy of Scientific and Innovative Research (AcSIR), New Delhi, 110025 India.
International Journal of Biological Macromolecules
|May 16, 2017
Summary
A novel DNA chip sensor rapidly identifies Streptococcus pyogenes, a common cause of infections. This ultrasensitive biosensor offers quick and specific pathogen detection from patient samples.
Area of Science:
- Biomedical Engineering
- Molecular Diagnostics
- Infectious Disease Detection
Background:
- Streptococcus pyogenes is a prevalent human pathogen causing diverse infections, from pharyngitis to rheumatic heart disease.
- Rapid and accurate detection of S. pyogenes is crucial for timely and effective disease treatment.
Purpose of the Study:
- To develop an ultrasensitive DNA chip-based sensor for the rapid identification of Streptococcus pyogenes.
- To enable quick detection of the pathogen directly from patient throat swab samples.
Main Methods:
- Development of a DNA chip sensor utilizing specific DNA probes.
- Measurement of amperometric response upon hybridization with single-stranded genomic DNA (ssG-DNA).
- Characterization using FTIR and SEM, and validation with clinical samples.
Main Results:
- The DNA chip sensor demonstrated high sensitivity (951.34 (μA/cm²) /ng DNA) and a low limit of detection (130 fg/6 μL).
- The sensor achieved highly specific identification of S. pyogenes.
- Pathogen identification was completed within 30 minutes.
Conclusions:
- The developed DNA chip-based sensor provides a rapid, ultrasensitive, and specific method for identifying Streptococcus pyogenes.
- This technology holds potential for quickPoint-of-care diagnosis of S. pyogenes infections.
