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Fluorescent Nanoparticles with Tissue-Dependent Affinity for Live Zebrafish Imaging.

Deepak Kumar Khajuria1, Vijay Bhooshan Kumar2, David Karasik1

  • 1The Musculoskeletal Genetics Laboratory, Faculty of Medicine in the Galilee, The Musculoskeletal Genetics Laboratory, Bar-Ilan University , Safed 1311502, Israel.

ACS Applied Materials & Interfaces
|May 16, 2017
PubMed
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Nitrogen-doped carbon quantum dots (N@CDs) show low toxicity and stable fluorescence in zebrafish, indicating potential for biological imaging. Their distribution suggests applications in studying lipid transport and diagnosing eye and skin defects.

Area of Science:

  • Biomedical Engineering
  • Materials Science
  • Nanotechnology

Background:

  • Carbon quantum dots (CDs) are explored for biomedical uses due to low toxicity and good solubility.
  • Fluorescent nitrogen-doped CDs (N@CDs) require further in vivo studies for their imaging potential.
  • Zebrafish (ZF) offer a valuable model for assessing in vivo toxicity and biodistribution of nanomaterials.

Purpose of the Study:

  • To develop a cost-effective synthesis for N@CDs.
  • To evaluate the in vivo toxicity and fluorescence imaging capabilities of N@CDs using zebrafish.
  • To explore potential applications of N@CDs in biological imaging and diagnostics.

Main Methods:

  • One-pot synthesis of nitrogen-doped carbon quantum dots (N@CDs).
  • In vivo administration of N@CDs to zebrafish embryos and larvae.
Keywords:
embryofluorescencelarvaelive imagingnanoparticleszebrafish

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  • Fluorescence microscopy to track N@CD distribution and assess toxicity.
  • Analysis of N@CD accumulation in specific tissues like the digestive system, eyes, and melanophores.

    • Main Results:

    • Successful low-cost, one-pot synthesis of N@CDs.
    • Stable, long-lasting fluorescence emission observed in zebrafish, confirming N@CD distribution.
    • No detectable toxicity of N@CDs in the zebrafish model.
    • N@CDs accumulated in the digestive system, suggesting potential for studying lipid metabolism.
    • High affinity for melanin observed, with accumulation in eyes and melanophore strips.

    Conclusions:

    • N@CDs are non-toxic and suitable for in vivo fluorescence imaging in zebrafish.
    • N@CDs show promise for imaging lipoprotein and nutritional biology via the digestive system.
    • The melanin-binding affinity of N@CDs suggests novel applications in eye and skin imaging and defect diagnosis.