Matrix solid-phase dispersion as a tool for phytochemical and bioactivities characterisation: Crataegus oxyacantha...

Area of Science:

• Phytochemistry
• Natural Product Extraction
• Analytical Chemistry

Background:

• Hawthorn (Crataegus oxyacantha L.) is a shrub with a rich phytochemical profile.
• Polyphenols are key bioactive compounds with significant health benefits.
• Traditional extraction methods can be time-consuming and solvent-intensive.

Purpose of the Study:

• To evaluate matrix solid-phase dispersion (MSPD) for polyphenol extraction from hawthorn.
• To characterize the phenolic profile of hawthorn extracts.
• To assess the bioactivity of hawthorn extracts obtained via MSPD.

Main Methods:

• Matrix solid-phase dispersion (MSPD) was employed for polyphenol extraction from hawthorn fruits and leaves.
• High-Performance Liquid Chromatography with Diode Array Detection (HPLC-DAD) was used for polyphenol identification.
• Antioxidant activity assays were performed on the extracts.

Main Results:

• MSPD extracts demonstrated high polyphenol content, with profiles varying by solvent.
• HPLC-DAD analysis identified major bioactive polyphenols, including novel compounds for the species.
• Hawthorn extracts exhibited remarkable antioxidant activity.

Conclusions:

• MSPD is an effective, fast, and environmentally friendly alternative for extracting polyphenols from hawthorn.
• The method allows for the discovery of new bioactive compounds.
• Hawthorn extracts possess significant antioxidant potential.