Biological response of commercially available different tricalcium silicate-based cements and pozzolan cement

This study evaluated four root repair materials for cytotoxicity and cell attachment in vitro. Cell viability was determined at 24 hr, 3 days and 7 days by using a 2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide (XTT) assay with material extracts. Cell adhesion was examined with a scanning electron microscope on the surface of materials at 24 and 48 hr. Angelus MTA displayed the lowest cell viabilities at all-day incubations. Endocem had high biocompatibility on the first day. After culturing for 3 days and 7 days, the cell viabilities of Biodentine, Endocem and MM-MTA had similar results, and their cell viability was significantly higher than that of Angelus. No definite relation was found between the incubation time and the relative cell viability in any group. In scanning electron micrographs, the cells were attached to the material surface for all materials, although the cells in the Biodentine group were attached better than the other groups on the second day. Cell viability and cell attachment was lower in the Angelus group. Endocem, Biodentine and MM-MTA were similar in biocompatibility and cytotoxicity.