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Multiple subelements within the polyomavirus enhancer function synergistically to activate DNA replication.

W J Muller1, D Dufort, J A Hassell

  • 1Department of Microbiology and Immunology, McGill University, Montreal, Quebec, Canada.

Molecular and Cellular Biology
|November 1, 1988
PubMed
Summary
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Polyomavirus DNA replication relies on an origin core and enhancer elements (alpha and beta). These enhancer elements, composed of core and auxiliary subelements, cooperatively bind cellular factors to activate replication.

Area of Science:

  • Molecular Biology
  • Virology
  • Genetics

Background:

  • The polyomavirus origin of DNA replication has distinct cis-acting components essential for its function.
  • These components include an origin core for replication and enhancer elements (alpha and beta) for both transcription and replication.

Purpose of the Study:

  • To identify the specific sequences within the polyomavirus enhancer elements (alpha and beta).
  • To understand how these sequences contribute to the activation of DNA replication and transcription.

Main Methods:

  • In vitro mutagenesis of polyomavirus enhancer elements (alpha and beta).
  • Assay of replication activation in cis of the origin core in MOP-8 cells.
  • Analysis of subelement function and cooperativity.

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Main Results:

  • The beta enhancer element consists of a core subelement and two auxiliary subelements, which act synergistically.
  • The alpha enhancer element also comprises a core and an auxiliary subelement.
  • Subelements appear functionally equivalent, with dimers of the core subelement showing significant activity.

Conclusions:

  • The polyomavirus enhancer exhibits a hierarchical organization: multiple subelements form enhancer elements, and multiple elements form the enhancer.
  • Distinct cellular factors bind to these subelements, suggesting cooperative action for polyomavirus DNA replication.
  • A common mechanism involving multiple cellular proteins likely activates polyomavirus DNA replication.