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Antitubercular Activity of Mycelium-Associated Ganoderma Lanostanoids
Masahiko Isaka1, Panida Chinthanom1, Malipan Sappan1
1National Center for Genetic Engineering and Biotechnology (BIOTEC) , 113 Thailand Science Park, Phaholyothin Road, Klong Luang, Pathumthani 12120, Thailand.
Journal of Natural Products
|May 16, 2017
Summary
Researchers explored lanostane triterpenoids from Ganoderma fungi for tuberculosis treatment. They identified new compounds and found specific acetoxy groups are crucial for antimycobacterial activity against Mycobacterium tuberculosis.
Area of Science:
- Natural Product Chemistry
- Medicinal Chemistry
- Mycology
Background:
- Ganoderma species are a known source of bioactive compounds.
- Lanostane triterpenoids have shown potential in previous antitubercular research.
- Submerged cultures offer a method for isolating fungal metabolites.
Purpose of the Study:
- To isolate and characterize new lanostane triterpenoids from Ganoderma species.
- To evaluate the antitubercular activity of isolated compounds against Mycobacterium tuberculosis.
- To establish structure-activity relationships for antimycobacterial effects.
Main Methods:
- Investigation of three Ganoderma strains (G. orbiforme, Ganoderma sp., G. australe) using submerged cultures.
- Isolation and structural elucidation of fourteen new and thirty-five known lanostane triterpenoids.
- Antitubercular activity screening of compounds against Mycobacterium tuberculosis H37Ra.
Main Results:
- Fourteen novel and thirty-five known lanostane triterpenoids were successfully isolated.
- Antitubercular activity was evaluated for the isolated compounds.
- 3β- and 15α-acetoxy groups were identified as critical for antimycobacterial activity.
Conclusions:
- Specific lanostane triterpenoids from Ganoderma species possess significant antimycobacterial properties.
- The presence of 3β- and 15α-acetoxy groups is essential for potent activity against Mycobacterium tuberculosis.
- (24E)-3β,15α-diacetoxylanosta-7,9(11),24-trien-26-oic acid (35) was identified as the most potent compound.
