Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nervous Tissue: Glial Cells01:31

Nervous Tissue: Glial Cells

7.6K
Glia, or neuroglia, are vital support cells that assist neurons in their functions. The term "glia" originates from the Greek word for "glue," reflecting their role in holding the nervous system together. These cells can be categorized into six types: four in the central nervous system (CNS) and two in the peripheral nervous system (PNS).
The CNS glial cell includes the astrocytes, the oligodendrocytes, the microglia, and the ependymal cells.
Astrocytes are star-shaped glial...
7.6K
Neurogenesis and Regeneration of Nervous Tissue01:15

Neurogenesis and Regeneration of Nervous Tissue

1.9K
In the CNS, neurogenesis, the birth of new neurons from stem cells, is limited to the hippocampus in adults. In other regions of the brain and spinal cord, neurogenesis is almost non-existent due to inhibitory influences from neuroglia, especially oligodendrocytes, and the absence of growth-stimulating cues. The myelin produced by oligodendrocytes in the CNS inhibits neuronal regeneration. Furthermore, astrocytes proliferate rapidly after neuronal damage, forming scar tissue that physically...
1.9K
Glial Cells01:04

Glial Cells

95.8K
Overview
95.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

First Successful Bloodless Combined Heart-Liver Transplantation in a Jehovah's Witness.

Clinical transplantation·2026
Same author

Imbalanced neurogenesis and gliogenesis in the developing neocortex of mice lacking the proteoglycan Tsukushi.

Developmental biology·2026
Same author

Long-Term Outcomes of Kidney Transplantation With Inferior Vena Cava Outflow: A Single Center Experience.

Transplantation proceedings·2026
Same author

Modest Neurodevelopment Impacts of <italic>APOE4</italic> in a Human Brain Organoid Model of Low-Grade SARS-CoV-2 Infection.

Developmental neuroscience·2026
Same author

Turnover of NESTIN-Negative Neural Progenitors Into NESTIN-Positive State by the Lack of JMJD3.

Genes to cells : devoted to molecular & cellular mechanisms·2026
Same author

Histone methyltransferase Setd8 preserves chromatin accessibility to safeguard retinal progenitor cell identity during development.

Stem cell reports·2026

Related Experiment Video

Updated: Mar 2, 2026

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
07:25

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Published on: July 7, 2022

3.4K

NEUROD1 Instructs Neuronal Conversion in Non-Reactive Astrocytes.

Rebecca Brulet1, Taito Matsuda2, Ling Zhang1

  • 1Departments of Molecular Biology, Neurology and Neurotherapeutics, and Hamon Center for Regenerative Science and Medicine, UT Southwestern Medical Center, Dallas, TX 75390, USA.

Stem Cell Reports
|May 17, 2017
PubMed
Summary

Researchers converted non-neuronal astrocytes into neurons using the NEUROD1 factor without brain injury. This astrocyte-to-neuron transdifferentiation occurred under physiological conditions, offering potential future clinical applications.

Keywords:
AAV9NeuroDastrocytescortexneuronsreprogrammingstriatumtransdifferentiation

More Related Videos

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
11:42

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Published on: June 10, 2021

5.5K
Isolation and Culture of Mouse Cortical Astrocytes
11:25

Isolation and Culture of Mouse Cortical Astrocytes

Published on: January 19, 2013

94.6K

Related Experiment Videos

Last Updated: Mar 2, 2026

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes
07:25

Isolation and Direct Neuronal Reprogramming of Mouse Astrocytes

Published on: July 7, 2022

3.4K
In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes
11:42

In vitro Modeling for Neurological Diseases using Direct Conversion from Fibroblasts to Neuronal Progenitor Cells and Differentiation into Astrocytes

Published on: June 10, 2021

5.5K
Isolation and Culture of Mouse Cortical Astrocytes
11:25

Isolation and Culture of Mouse Cortical Astrocytes

Published on: January 19, 2013

94.6K

Area of Science:

  • Neuroscience
  • Cell Biology
  • Regenerative Medicine

Background:

  • Current methods for neuronal conversion require brain injury.
  • The potential for neuronal transdifferentiation long after injury is not well understood.
  • Astrocyte-to-neuron conversion is a key area for brain repair research.

Purpose of the Study:

  • To investigate astrocyte-to-neuron transdifferentiation under physiological conditions.
  • To determine if NEUROD1 can induce neuronal conversion without causing brain damage.
  • To explore the feasibility of neuronal conversion in non-reactive astrocytes.

Main Methods:

  • Utilized adeno-associated virus 9 (AAV9) for intravascular delivery of the NEUROD1 transcription factor.
  • Administered AAV9 to cross the blood-brain barrier without inducing injury.
  • Examined neuronal conversion in both non-reactive astrocytes and cultured astrocytes with minimized proliferation.

Main Results:

  • A small but significant number of non-reactive astrocytes successfully converted to neurons in the striatum.
  • Neuronal transdifferentiation was not observed in the cortex under these conditions.
  • Astrocytes with reduced proliferative potential showed limited NEUROD1-induced neuronal conversion.

Conclusions:

  • A single transcription factor, NEUROD1, can induce astrocyte-to-neuron conversion under physiological conditions.
  • This conversion can occur without direct brain injury, utilizing a non-invasive delivery method.
  • Findings suggest potential for future clinical strategies for neurological repair long after injury phases.